ArticleOncogene2003
Immediate-early gene induction by the stresses anisomycin and arsenite in human osteosarcoma cells involves MAPK cascade signaling to Elk-1, CREB and SRF.
Article in Oncogene, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 62 citations in OpenAlex.
- Transcriptome of Cultured Lung Fibroblasts in Idiopathic Pulmonary Fibrosis: Meta-Analysis of Publically Available Microarray Datasets Reveals Repression of Inflammation and Immunity Pathways.International journal of molecular sciences · 2016Pooled it
- Mechanistic Insights and Clinical Implications of ELK1 in Solid Tumors: A Narrative Review.Cells · 2025Review
- Hesperetin but not ellagic acid increases myosin heavy chain expression and cell fusion in C2C12 myoblasts in the presence of oxidative stress.Frontiers in nutrition · 2024Article
- The impact of signaling pathways on the desmosome ultrastructure in pemphigus.Frontiers in immunology · 2024Article
- Modulation of the p38 MAPK Pathway by Anisomycin Promotes Ferroptosis of Hepatocellular Carcinoma through Phosphorylation of H3S10.Oxidative medicine and cellular longevity · 2022Article
- Cardiac glycoside/aglycones inhibit HIV-1 gene expression by a mechanism requiring MEK1/2-ERK1/2 signaling.Scientific reports · 2018Article
- Partial p53-dependence of anisomycin-induced apoptosis in PC12 cells.Molecular and cellular biochemistry · 2017Article
- Article
- microRNA let-7c is essential for the anisomycin-elicited apoptosis in Jurkat T cells by linking JNK1/2 to AP-1/STAT1/STAT3 signaling.Scientific reports · 2016Article
- Article
- Breast cancer adaptive resistance: HER2 and cancer stem cell repopulation in a heterogeneous tumor society.Journal of cancer research and clinical oncology · 2014Review
- Inositol polyphosphate multikinase is a coactivator for serum response factor-dependent induction of immediate early genes.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Impairment of FOS mRNA stabilization following translation arrest in granulocytes from myelodysplastic syndrome patients.PloS one · 2013Article
- Transfer of neuroplasticity from nucleus accumbens core to shell is required for cocaine reward.PloS one · 2012Article
- Stress induced gene expression: a direct role for MAPKAP kinases in transcriptional activation of immediate early genes.Nucleic acids research · 2011Article
- The G2 p38-mediated stress-activated checkpoint pathway becomes attenuated in transformed cells.Current biology : CB · 2007Article
- Specific inhibitor of MEK-mediated cross-talk between ERK and p38 MAPK during differentiation of human osteosarcoma cells.Journal of cell communication and signaling · 2007Article
- Insulin-like growth factors 1 and 2 induce lymphangiogenesis in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Co-activation of ERK, NF-kappaB, and GADD45beta in response to ionizing radiation.The Journal of biological chemistry · 2005Article
- Retinoic acid and arsenic trioxide cooperate for apoptosis through phosphorylated RXR alpha.Oncogene · 2005Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cellular stress activates multiple mitogen-activated protein kinase (MAPK) cascades and immediate-early gene (IEG) transcription. To address how these events are linked, we investigated the endogenous signaling/transcription factor network driving IEG activation by arsenite and anisomycin in the human osteosarcoma cell line HOS/TE-85. Induction of IEG transcription by both stresses corresponded temporally with the phosphorylation of the regulatory factors Elk-1 and cAMP response element-binding protein (CREB), along with activation of the extracellular signal-regulated kinase (ERK), stress-activated protein kinase (SAPK) and p38 MAPK cascades. To assess the role of the different cascades, they were selectively inhibited with PD98059, SP600125 and SB203580, respectively. This implicated all three cascades in Elk-1 phosphorylation after arsenite treatment, whereas ERK and SAPK inhibition diminished this, and IEG mRNA levels, downstream of anisomycin. SB blocked phosphorylation of both serum response factor (SRF) and CREB, and strongly reduced IEG activation by both stresses. Combining PD with SB further reduced arsenite induction of IEG transcription. Thus, all three MAPK cascades mediate anisomycin- and arsenite-induced signaling to IEG promoters in HOS cells through the differential targeting of Elk-1, SRF and CREB.
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