ArticleThe EMBO journal2003
Stop codon selection in eukaryotic translation termination: comparison of the discriminating potential between human and ciliate eRF1s.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 34 citations in OpenAlex.
- Genetic analysis of L123 of the tRNA-mimicking eukaryote release factor eRF1, an amino acid residue critical for discrimination of stop codons.Nucleic acids research · 2015Article
- New insights into stop codon recognition by eRF1.Nucleic acids research · 2015Article
- Therapeutics based on stop codon readthrough.Annual review of genomics and human genetics · 2014Review
- Two-step model of stop codon recognition by eukaryotic release factor eRF1.Nucleic acids research · 2013Article
- A single amino acid change of translation termination factor eRF1 switches between bipotent and omnipotent stop-codon specificity.Nucleic acids research · 2011Article
- In vitro incorporation of nonnatural amino acids into protein using tRNA(Cys)-derived opal, ochre, and amber suppressor tRNAs.RNA (New York, N.Y.) · 2010Article
- Translation termination in pyrrolysine-utilizing archaea.FEBS letters · 2009Article
- Molecular dissection of translation termination mechanism identifies two new critical regions in eRF1.Nucleic acids research · 2009Article
- Different modes of stop codon restriction by the Stylonychia and Paramecium eRF1 translation termination factors.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Distinct paths to stop codon reassignment by the variant-code organisms Tetrahymena and Euplotes.Molecular and cellular biology · 2006Article
- Sequencing of random Euplotes crassus macronuclear genes supports a high frequency of +1 translational frameshifting.Eukaryotic cell · 2005Article
- Decoding the decoding region: analysis of eukaryotic release factor (eRF1) stop codon-binding residues.Journal of molecular evolution · 2005Article
- Backbone (1)H, (13)C and (15)N resonance assignment of the N-terminal domain of human eRF1.Journal of biomolecular NMR · 2004Article
- GTP hydrolysis by eRF3 facilitates stop codon decoding during eukaryotic translation termination.Molecular and cellular biology · 2004Article
- Antizyme frameshifting as a functional probe of eukaryotic translational termination.Nucleic acids research · 2003Article
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During eukaryotic translation termination, eRF1 responds to three stop codons. However, in ciliates with variant genetic codes, only one or two codons function as a stop signal. To localize the region of ciliate eRF1 implicated in stop codon discrimination, we have constructed ciliate-human hybrid eRF1s by swapping regions of human eRF1 for the equivalent region of ciliate Euplotes eRF1. We have examined the formation of a cross-link between recombinant eRF1s and mRNA analogs containing the photoactivable 4-thiouridine (s(4)U) at the first position of stop and control sense codons. With human eRF1, this cross-link can be detected only when either stop or UGG codons are located in the ribosomal A site. Here we show that the cross-link of the Euplotes-human hybrid eRF1 is restricted to mRNAs containing UAG and UAA codons, and that the entire N-terminal domain of Euplotes eRF1 is involved in discriminating against UGA and UGG. On the basis of these results, we discuss the steps of the selection process that determine the accuracy of stop codon recognition in eukaryotes.
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