ArticleProceedings of the National Academy of Sciences of the United States of America2003
Probing polymerization forces by using actin-propelled lipid vesicles.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 191 citations in OpenAlex.
- On the Assembly of Actin Polymerization-Powered Motors.Macromolecular rapid communications · 2026Article
- Article
- A Microfluidic Platform for Actin-Based Membrane Remodeling Reveals the Stabilizing Role of Branched Actin Networks on Lipid Microdomains.Small science · 2025Article
- Reconstituted systems for studying the architecture and dynamics of actin networks.The Biochemical journal · 2025Review
- Mechanical control of antigen detection and discrimination by T and B cell receptors.Biophysical journal · 2024Review
- ActuAtor, a Listeria-inspired molecular tool for physical manipulation of intracellular organizations through de novo actin polymerization.Cell reports · 2023Article
- Studying actin-induced cell shape changes using Giant Unilamellar Vesicles and reconstituted actin networks.Biochemical Society transactions · 2022Review
- Encapsulated bacteria deform lipid vesicles into flagellated swimmers.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A computational modeling of invadopodia protrusion into an extracellular matrix fiber network.Scientific reports · 2022Article
- On the adhesion-velocity relation and length adaptation of motile cells on stepped fibronectin lanes.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Pattern Formation and Complexity in Single Cells.Current biology : CB · 2020Review
- Cytoskeleton Force Exertion in Bulk Cytoplasm.Frontiers in cell and developmental biology · 2020Review
- Pulling-force generation by ensembles of polymerizing actin filaments.Physical biology · 2019Article
- Actin Dynamics Drive Microvillar Motility and Clustering during Brush Border Assembly.Developmental cell · 2019Article
- Creation of Artificial Cell-Like Structures Promoted by Microfluidics Technologies.Micromachines · 2019Review
- Molecular mechanisms of force production in clathrin-mediated endocytosis.FEBS letters · 2018Review
- Light-induced propulsion of a giant liposome driven by peptide nanofibre growth.Scientific reports · 2018Article
- Artificial cell mimics as simplified models for the study of cell biology.Experimental biology and medicine (Maywood, N.J.) · 2017Review
- Micrometer-Scale Membrane Transition of Supported Lipid Bilayer Membrane Reconstituted with Cytosol of Dictyostelium discoideum.Life (Basel, Switzerland) · 2017Article
- Toward the reconstitution of synthetic cell motility.Cell adhesion & migration · 2016Review
12 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Actin polymerization provides a powerful propulsion force for numerous types of cell motility. Although tremendous progress has been made in identifying the biochemical components necessary for actin-based motility, the precise biophysical mechanisms of force generation remain unclear. To probe the polymerization forces quantitatively, we introduce an experimental system in which lipid vesicles coated with the Listeria monocytogenes virulence factor ActA are propelled by actin polymerization. The polymerization forces cause significant deformations of the vesicle. We have used these deformations to obtain a spatially resolved measure of the forces exerted on the membrane using a model based on the competition between osmotic pressure and membrane stretching. Our results indicate that actin exerts retractile or propulsive forces depending on the local membrane curvature and that the membrane is strongly bound to the actin gel. These results are consistent with the observed dynamics. After a slow elongation of the vesicle from a spherical shape, the strong bonds between the actin gel and the membrane rupture if the retractile forces exceed a critical value, leading to a rapid release of the vesicle's trailing edge.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.