ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2003
Direct cAMP signaling through G-protein-coupled receptors mediates growth cone attraction induced by pituitary adenylate cyclase-activating polypeptide.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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The trial behind it
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Who cites it
45 citing papers in PubMed, 78 citations in OpenAlex.
- Protective Effects of PACAP in Diabetic Complications: Retinopathy, Nephropathy and Neuropathy.International journal of molecular sciences · 2025Review
- Dysfunctional purinergic signaling correlates with disease severity in COVID-19 patients.Frontiers in immunology · 2022Article
- Pituitary Adenylate Cyclase-Activating Polypeptide: A Promising Neuroprotective Peptide in Stroke.Aging and disease · 2020Review
- Molecular and cellular correlates of human nerve regeneration: ADCYAP1/PACAP enhance nerve outgrowth.Brain : a journal of neurology · 2020Article
- Review on PACAP-Induced Transcriptomic and Proteomic Changes in Neuronal Development and Repair.International journal of molecular sciences · 2018Review
- Cyclic Nucleotide Control of Microtubule Dynamics for Axon Guidance.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Optogenetic activation of axon guidance receptors controls direction of neurite outgrowth.Scientific reports · 2016Article
- Differential requirement of F-actin and microtubule cytoskeleton in cue-induced local protein synthesis in axonal growth cones.Neural development · 2015Article
- Structural and morphometric comparison of the molar teeth in pre-eruptive developmental stage of PACAP-deficient and wild-type mice.Journal of molecular neuroscience : MN · 2014Article
- Injury-associated PACAP expression in rat sensory and motor neurons is induced by endogenous BDNF.PloS one · 2014Article
- Cell biology in neuroscience: RNA-based mechanisms underlying axon guidance.The Journal of cell biology · 2013Review
- VIP and PACAP: neuropeptide modulators of CNS inflammation, injury, and repair.British journal of pharmacology · 2013Review
- Role of mitochondrial activation in PACAP dependent neurite outgrowth.Journal of molecular neuroscience : MN · 2012Article
- Neural Explant Cultures from Xenopus laevis.Journal of visualized experiments : JoVE · 2012Article
- Signaling through the neuropeptide GPCR PAC₁ induces neuritogenesis via a single linear cAMP- and ERK-dependent pathway using a novel cAMP sensor.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- Microtubule redistribution in growth cones elicited by focal inactivation of kinesin-5.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Axonal mRNA localization and local protein synthesis in nervous system assembly, maintenance and repair.Nature reviews. Neuroscience · 2012Review
- Concentration-dependent requirement for local protein synthesis in motor neuron subtype-specific response to axon guidance cues.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Second messengers and membrane trafficking direct and organize growth cone steering.Nature reviews. Neuroscience · 2011Review
- Use of laser microdissection in the investigation of facial motoneuron and neuropil molecular phenotypes after peripheral axotomy.Experimental neurology · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Developing axons are guided to their appropriate targets by environmental cues through the activation of specific receptors and intracellular signaling pathways. Here we report that gradients of pituitary adenylate cyclase-activating polypeptide (PACAP), a neuropeptide widely expressed in the developing nervous system, induce marked attraction of Xenopus growth cones in vitro. PACAP exerted its chemoattractive effects through PAC1, a PACAP-selective G-protein-coupled receptor (GPRC) expressed at the growth cone. Furthermore, the attraction depended on localized cAMP signaling because it was completely blocked either by global elevation of intracellular cAMP levels using forskolin or by inhibition of protein kinase A using specific inhibitors. Moreover, local direct elevation of intracellular cAMP by focal photolysis of caged cAMP compounds was sufficient to induce growth cone attraction. On the other hand, blockade of Ca2+, phospholipase C, or phosphatidyl inositol-3 kinase signaling pathways did not affect PACAP-induced growth cone attraction. Finally, PACAP-induced attraction also involved the Rho family of small GTPases and required local protein synthesis. Taken together, our results establish cAMP signaling as an independent pathway capable of mediating growth cone attraction induced by a physiologically relevant peptide acting through GPCRs. Such a direct cAMP pathway could potentially operate in other guidance systems for the accurate wiring of the nervous system.
Indexed as
Identifiers
12657686PMC6742047W2118578503What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.