ArticleMolecular genetics and genomics : MGG2003
Construction of a BAC library and generation of BAC end sequence-tagged connectors for genome sequencing of the African malaria mosquito Anopheles gambiae.
Article in Molecular genetics and genomics : MGG, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- The Use of Lupin as a Source of Protein in Animal Feeding: Genomic Tools and Breeding Approaches.International journal of molecular sciences · 2019Review
- Preparation of megabase-sized DNA from a variety of organisms using the nuclei method for advanced genomics research.Nature protocols · 2012Article
- Construction of BIBAC and BAC libraries from a variety of organisms for advanced genomics research.Nature protocols · 2012 · on this mapArticle
- HTS-PEG: a method for high throughput sequencing of the paired-ends of genomic libraries.PloS one · 2012Article
- The genome sequence of the North-European cucumber (Cucumis sativus L.) unravels evolutionary adaptation mechanisms in plants.PloS one · 2011Article
- Two duplicated P450 genes are associated with pyrethroid resistance in Anopheles funestus, a major malaria vector.Genome research · 2009Article
- End-sequencing and characterization of silkworm (Bombyx mori) bacterial artificial chromosome libraries.BMC genomics · 2007Article
- Segmental duplication implicated in the genesis of inversion 2Rj of Anopheles gambiae.PloS one · 2007Article
- Update of the Anopheles gambiae PEST genome assembly.Genome biology · 2007Article
- Construction and characterization of BAC libraries from major grapevine cultivars.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- A highly conserved candidate chemoreceptor expressed in both olfactory and gustatory tissues in the malaria vector Anopheles gambiae.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Isolation and characterization of Y chromosome sequences from the African malaria mosquito Anopheles gambiae.Genetics · 2004Article
- High-resolution genetic and physical mapping of the cauliflower high-beta-carotene gene Or ( Orange).Molecular genetics and genomics : MGG · 2003Article
- Quantitative trait loci in Anopheles gambiae controlling the encapsulation response against Plasmodium cynomolgi Ceylon.BMC genetics · 2003Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 1 country.
Funding
Abstract
A Bacterial Artificial Chromosome (BAC) genomic DNA library of Anopheles gambiae, the major human malaria vector in sub-Saharan Africa, was constructed and characterized. This library (ND-TAM) is composed of 30,720 BAC clones in eighty 384-well plates. The estimated average insert size of the library is 133 kb, with an overall genome coverage of approximately 14-fold. The ends of approximately two-thirds of the clones in the library were sequenced, yielding 32,340 pair-mate ends. A statistical analysis (G-test) of the results of PCR screening of the library indicated a random distribution of BACs in the genome, although one gap encompassing the white locus on the X-chromosome was identified. Furthermore, combined with another previously constructed BAC library (ND-1), ~2,000 BACs have been physically mapped by polytene chromosomal in situ hybridization. These BAC end pair mates and physically mapped BACs have been useful for both the assembly of a fully sequenced A. gambiae genome and for linking the assembled sequence to the three polytene chromosomes. This ND-TAM library is now publicly available at both http://www.malaria.mr4.org/mr4pages/index.html/ and http://hbz.tamu.edu/, providing a valuable resource to the mosquito research community.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.