ArticleMolecular genetics and genomics : MGG2003
The Aspergillus parasiticus protein AFLJ interacts with the aflatoxin pathway-specific regulator AFLR.
Article in Molecular genetics and genomics : MGG, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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71 citing papers in PubMed.
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- Identification and Characterization of a Red-Light Sensor FphA inInternational journal of molecular sciences · 2026Article
- Chromatin Remodeling Factor Arp8 Is Involved in the Regulation of Aflatoxin Biosynthesis and Fungal Pathogenicity ofJournal of fungi (Basel, Switzerland) · 2026Article
- From the bench to the reactor: engineered filamentous fungi for biochemical and biomaterial production.Biotechnology for biofuels and bioproducts · 2025Review
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- Aspergillus Mycotoxins: The Major Food Contaminants.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Synergistic inhibition ofFrontiers in veterinary science · 2025Article
- SntB triggers the antioxidant pathways to regulate development and aflatoxin biosynthesis ineLife · 2024Article
- Comparative Genome Analysis of Japanese Field-IsolatedJournal of fungi (Basel, Switzerland) · 2024Article
- Aspergillus flavus pangenome (AflaPan) uncovers novel aflatoxin and secondary metabolite associated gene clusters.BMC plant biology · 2024Article
- Aflatoxin profiles of Aspergillus flavus isolates in Sudanese fungal rhinosinusitis.Medical mycology · 2024Article
- Targeting of Specialized Metabolites Biosynthetic Enzymes to Membranes and Vesicles by Posttranslational Palmitoylation: A Mechanism of Non-Conventional Traffic and Secretion of Fungal Metabolites.International journal of molecular sciences · 2024Review
- An environmental isolate ofFrontiers in microbiology · 2024Article
- Structure-guided discovery of novel AflG inhibitors for aflatoxin contamination control inFrontiers in microbiology · 2024Article
- Filters of automobile air conditioning systems as in-car source of exposure to infections and toxic moulds.Environmental science and pollution research international · 2023Article
- Discovery of long non-coding RNAs in Aspergillus flavus response to water activity, COScientific reports · 2023Article
- Current Insights in Fungal Importance-A Comprehensive Review.Microorganisms · 2023Review
- Biodegradation of Aflatoxin B1 in Maize Grains and Suppression of Its Biosynthesis-Related Genes Using EndophyticJournal of fungi (Basel, Switzerland) · 2023Article
- Genetic Regulation of Mycotoxin Biosynthesis.Journal of fungi (Basel, Switzerland) · 2022Review
- Histone 2-Hydroxyisobutyryltransferase Encoded byToxins · 2022Article
11 more citing papers are in PubMed but not listed here.
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Abstract
The Aspergillus parasiticus aflJgene, which is located in the aflatoxin biosynthetic gene cluster and is transcribed divergently from the aflatoxin pathway regulatory gene aflR, encodes a 438-amino acid protein. Transformation with aflJplus aflR, but not aflJalone, increased the accumulation of aflatoxin precursors substantially in an O-methylsterigmatocystin-accumulating A. parasiticus strain. Disruption of aflJ resulted in non-pigmented mutants that lost the ability to synthesize aflatoxin intermediates. Transcript profiling by real time RT-PCR indicated that the lack of aflJtranscripts in the aflJ knockout mutants is associated with a significant decrease in the transcript levels of the genes for early (pksAand nor1), middle (ver1) and later (omtA) steps in the aflatoxin biosynthetic pathway, with the degree of reduction ranging from 5- to 20-fold. Deletion of aflJ, however, did not have any effect on the aflRtranscript level, and vice versa. Two-hybrid assays showed that AFLJ did not interact with aflatoxin biosynthetic enzymes, including NOR1, VER1, OMTA and ORDA. But AFLJ interacted with full-length AFLR, and the DNA-binding domain of AFLR was found not to be essential for this interaction. Simultaneous substitution of Arg427, Arg429, and Arg431 in the C-terminal region of AFLR with Leu residues abolished its ability to interact with AFLJ. Replacement of Asp436, which was previously shown to be crucial for AFLR's activation activity, with His, in contrast, had little effect on the interaction. On the other hand, deletions in most regions of AFLJ appeared to destroy its function, despite the fact that random amino acid substitution(s) in its C-terminal region did not drastically affect its capacity to interact with AFLR. The results show that aflJis involved in the expression of aflatoxin structural genes and support the hypothesis that aflJis a coactivator gene.
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