ArticleMolecular genetics and genomics : MGG2003
Effects of selective inactivation of individual genes for low-molecular-mass subunits on the assembly of photosystem II, as revealed by chloroplast transformation: the psbEFLJoperon in Nicotiana tabacum.
Article in Molecular genetics and genomics : MGG, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 81 citations in OpenAlex.
- The lumenal domain of Cyt bPhotosynthesis research · 2025Article
- Article
- Analysis of Plant Physiological Parameters and Gene Transcriptional Changes Under the Influence of Humic Acid and Humic Acid-Amino Acid Combinations in Maize.International journal of molecular sciences · 2024Article
- The biogenesis and maintenance of PSII: Recent advances and current challenges.The Plant cell · 2024Review
- RESISTANCE TO PHYTOPHTHORA1 promotes cytochrome b559 formation during early photosystem II biogenesis in Arabidopsis.The Plant cell · 2024Article
- Membrane protein provision controls prothylakoid biogenesis in tobacco etioplasts.The Plant cell · 2024Article
- Shedding light on photosystem II components in the dark.The Plant cell · 2024Article
- Modulation of antioxidant defense and PSII components by exogenously applied acetate mitigates salinity stress in Avena sativa.Scientific reports · 2024Article
- Comparative chloroplast genomes ofFrontiers in plant science · 2024Article
- The PsbJ protein is required for photosystem II activity in centers lacking the PsbO and PsbV lumenal subunits.Photosynthesis research · 2022Article
- New Structural and Mechanistic Insights Into Functional Roles of CytochromeFrontiers in plant science · 2022Review
- Tandem gene amplification restores photosystem II accumulation in cytochrome bThe New phytologist · 2022Article
- The availability of neither D2 nor CP43 limits the biogenesis of photosystem II in tobacco.Plant physiology · 2021Article
- The Arabidopsis mTERF-repeat MDA1 protein plays a dual function in transcription and stabilization of specific chloroplast transcripts within the psbE and ndhH operons.The New phytologist · 2020Article
- The Role of Chloroplast Gene Expression in Plant Responses to Environmental Stress.International journal of molecular sciences · 2020Review
- Mutation of YL Results in a Yellow Leaf with Chloroplast RNA Editing Defect in Soybean.International journal of molecular sciences · 2020Article
- Jasmonic acid and methyl jasmonate modulate growth, photosynthetic activity and expression of photosystem II subunit genes in Brassica oleracea L.Scientific reports · 2020Article
- The Low Molecular Mass Photosystem II Protein PsbTn Is Important for Light Acclimation.Plant physiology · 2019Article
- The Roles of Cytochrome b 559 in Assembly and Photoprotection of Photosystem II Revealed by Site-Directed Mutagenesis Studies.Frontiers in plant science · 2015Review
- PsbN is required for assembly of the photosystem II reaction center in Nicotiana tabacum.The Plant cell · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Photosystem (PSII) is a supramolecular polypeptide complex found in oxygenic photosynthetic membranes, which is capable of extracting electrons from water for the reduction of plastoquinone. An intriguing feature of this assembly is the fact that it includes more than a dozen low-mass polypeptides of generally unknown function. Using a transplastomic approach, we have individually disrupted the genes of the psbEFLJoperon in Nicotiana tabacum, which encode four such polypeptides, without impairing expression of downstream loci of the operon. All four mutants exhibited distinct phenotypes; none of them was capable of photoautotrophic growth. All mutants bleached rapidly in the light. Disruption of psbEand psbF, which code for the alpha and beta apoproteins of cytochrome b(559), abolished PSII activity, as expected; Delta psbL and Delta psbJ plants displayed residual PSII activity in young leaves. Controlled partial solubilisation of thylakoid membranes uncovered surprisingly severe impairment of PSII structure, with subunit and assembly patterns varying depending on the mutant considered. In the Delta psbL mutant PSII was assembled primarily in a monomeric form, the homodimeric form was preponderant in Delta psbJ, and, unlike the case in Delta psbZ, the thylakoids of both mutants released some PSII supercomplexes. On the other hand, Photosystem I (PSI), the cytochrome b(6)f complex, ATP synthase, LHCII, and CP24/CP26/CP29 antennae were present in near wild-type levels. The data are discussed in terms of their implications for structural, biogenetic and functional aspects of PSII.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.