ArticleProceedings of the National Academy of Sciences of the United States of America2003
Identification and functional characterization of a novel binding site on TNF-alpha promoter.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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Who cites it
29 citing papers in PubMed, 65 citations in OpenAlex.
- The effect of chronic, non-pathogenic maternal immune activation on offspring postnatal muscle and immune outcomes.Journal of animal science · 2024Article
- Kavain ablates the radio-resistance of IDH-wildtype glioblastoma by targeting LITAF/NF-κB pathway.Cellular oncology (Dordrecht, Netherlands) · 2023Article
- Article
- GILP family: a stress-responsive group of plant proteins containing a LITAF motif.Functional & integrative genomics · 2018Article
- The feedback loop of LITAF and BCL6 is involved in regulating apoptosis in B cell non-Hodgkin's-lymphoma.Oncotarget · 2016Article
- PIG7 promotes leukemia cell chemosensitivity via lysosomal membrane permeabilization.Oncotarget · 2016Article
- Kavain Inhibition of LPS-Induced TNF-α via ERK/LITAF.Toxicology research · 2016Article
- LPS-induced TNF-α factor mediates pro-inflammatory and pro-fibrogenic pattern in non-alcoholic fatty liver disease.Oncotarget · 2015Article
- SIMPLE: A new regulator of endosomal trafficking and signaling in health and disease.Communicative & integrative biology · 2013Article
- Article
- Regulation of anti-Plasmodium immunity by a LITAF-like transcription factor in the malaria vector Anopheles gambiae.PLoS pathogens · 2012Article
- Whole-body deletion of LPS-induced TNF-α factor (LITAF) markedly improves experimental endotoxic shock and inflammatory arthritis.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Developments on drug discovery and on new therapeutics: highly diluted tinctures act as biological response modifiers.BMC complementary and alternative medicine · 2011Article
- LITAF and TNFSF15, two downstream targets of AMPK, exert inhibitory effects on tumor growth.Oncogene · 2011Article
- Differential effects of Radix Paeoniae Rubra (Chishao) on cytokine and chemokine expression inducible by mycobacteria.Chinese medicine · 2011Article
- SIMPLE/LITAF expression induces the translocation of the ubiquitin ligase itch towards the lysosomal compartments.PloS one · 2011Article
- Article
- LITAF mediation of increased TNF-α secretion from inflamed colonic lamina propria macrophages.PloS one · 2011Article
- Beneficial dysregulation of the time course of inflammatory mediators in lipopolysaccharide-induced tumor necrosis factor alpha factor-deficient mice.Clinical and vaccine immunology : CVI · 2010Article
- A PTP4A3 peptide PIMAP39 modulates TNF-alpha levels and endotoxic shock.Journal of innate immunity · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Transcription of the tumor necrosis factor (TNF) gene is rapidly and transiently induced by lipopolysaccharide in cells of monocytemacrophage lineage. Previous studies have suggested that in the mouse, multiple NF-kappaBRel-binding sites contribute to the TNF transcriptional response to LPS. But the role of these regulatory elements in transcriptional activation of the TNF-alpha gene in human monocytes remains unclear. Previously, a transcription factor, termed lipopolysaccharide-induced TNF-alpha factor (LITAF), was found to regulate TNF-alpha gene expression. However, the specific protein domain(s) of human (h)LITAF that interact with the hTNF-alpha promoter had not been identified. In this study, we identify by footprinting a sequence motif, CTCCC (-515 to -511), within the TNF-alpha promoter that binds to hLITAF. We also identify the region of hLITAF (amino acids 165-180) that was named peptide B and specifically mediates binding to the hTNF-alpha promoter. When THP-1 cells were stimulated with this peptide B, it was sufficient to induce TNF-alpha secretion. Induction of TNF-alpha transcription by LPS or peptide B depended on the presence of the -515 to -511 promoter region, which was found to be essential for hLITAF binding. Together, these findings help to clarify the mechanism of hLITAFhTNF-alpha interaction and the manner by which hLITAF contributes to hTNF-alpha regulation in an attempt to design new pharmacological interventions to address TNF-related diseases.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.