ArticleNucleic acids research2003
A simple and cost-effective method for producing small interfering RNAs with high efficacy.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 72 citations in OpenAlex.
- Envisaging the role of plant Small RNAs: A comprehensive review on antiviral defense mechanisms.Molecular biology reports · 2026Review
- Chemically Modified DNAzyme with Enhanced Activity for Sensitive MicroRNA Imaging in Live Cells.Molecules (Basel, Switzerland) · 2026Article
- Targeting Undruggable Proteins: The siRNA Revolution Beyond Small Molecules - Advances, Challenges, and Future Prospects in Therapeutic Innovation.Current gene therapy · 2026Review
- RNA Interference Applied to Crustacean Aquaculture.Biomolecules · 2024Review
- Direct dsRNA preparation by promoter-free RCT and RNase H cleavage using one circular dsDNA template with a mismatched bubble.RNA (New York, N.Y.) · 2023Article
- Native RNA Purification Method for Small RNA Molecules Based on Asymmetrical Flow Field-Flow Fractionation.Pharmaceuticals (Basel, Switzerland) · 2022Article
- RNA Interference Strategies for Future Management of Plant Pathogenic Fungi: Prospects and Challenges.Plants (Basel, Switzerland) · 2021Article
- Multiple gene targeting siRNAs for down regulation of Immediate Early-2 (Ie2) and DNA polymerase genes mediated inhibition of novel rat Cytomegalovirus (strain All-03).Virology journal · 2020Article
- Vaginal siRNA delivery: overview on novel delivery approaches.Drug delivery and translational research · 2020Review
- Bacterium-Mediated RNA Interference: Potential Application in Plant Protection.Plants (Basel, Switzerland) · 2019Review
- RNA Interference as a Prospective Tool for the Control of Human Viral Infections.Frontiers in microbiology · 2018Review
- A Simple and Cost-Effective Approach for In Vitro Production of Sliced siRNAs as Potent Triggers for RNAi.Molecular therapy. Nucleic acids · 2017Article
- Preparation of Small RNAs Using Rolling Circle Transcription and Site-Specific RNA Disconnection.Molecular therapy. Nucleic acids · 2015Article
- Silencing heat shock protein 27 (HSP27) inhibits the proliferation and migration of vascular smooth muscle cells in vitro.Molecular and cellular biochemistry · 2014Article
- Article
- Cross-protective effect of antisense oligonucleotide developed against the common 3' NCR of influenza A virus genome.Molecular biotechnology · 2013Article
- RNA interference with special reference to combating viruses of crustacea.Indian journal of virology : an official organ of Indian Virological Society · 2012Article
- RNA interference targeting virion core protein ORF095 inhibits Goatpox virus replication in Vero cells.Virology journal · 2012Article
- Gene silencing of HPV16 E6/E7 induced by promoter-targeting siRNA in SiHa cells.British journal of cancer · 2009Article
- A significant increase of RNAi efficiency in human cells by the CMV enhancer with a tRNAlys promoter.Journal of biomedicine & biotechnology · 2009Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small interfering RNAs (siRNAs) are powerful RNA interference (RNAi) reagents for directed post- transcriptional gene silencing. Exogenous siRNA is frequently used in RNAi studies. However, due to profound differences in the activity of siRNAs targeted to different regions of a gene, several reagents may have to be screened for optimal activity. This approach is expensive due to the cost of chemical synthesis of RNAs. We report a technically simple, quick and cost-effective method for the production of siRNAs that makes use of in vitro transcription and deoxyribozyme digestion of the transcripts to produce the desired sequence and length. The method allows for several siRNAs to be produced in parallel at much reduced costs. The siRNAs produced with this method were tested in MDA-MB-231 human breast cancer cells for efficacy against the type 1 insulin-like growth factor receptor (IGF1R) mRNA and they caused dose-dependent inhibition of IGF1R expression comparable to that induced by chemically synthesised siRNAs of the same sequence. This method is also useful for producing long RNA fragments of defined length and sequence that may be difficult to synthesise chemically, and also for producing large quantities of RNAs for applications including structural studies and the study of interactions between RNA and other molecules, such as proteins, other nucleic acids and drugs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.