ArticleNucleic acids research2003
Light-directed 5'-->3' synthesis of complex oligonucleotide microarrays.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- High-Efficiency Reverse (5'→3') Synthesis of Complex DNA Microarrays.Scientific reports · 2018Article
- Comprehensive transcriptome analysis of the periodontopathogenic bacterium Porphyromonas gingivalis W83.Journal of bacteriology · 2012Article
- Probing genomic diversity and evolution of Streptococcus suis serotype 2 by NimbleGen tiling arrays.BMC genomics · 2011Article
- Detection and identification of common food-borne viruses with a tiling microarray.The open virology journal · 2011Article
- Broad spectrum microarray for fingerprint-based bacterial species identification.BMC biotechnology · 2010Article
- An efficient algorithm for the stochastic simulation of the hybridization of DNA to microarrays.BMC bioinformatics · 2009Article
- Genomic diversity and evolution of Mycobacterium ulcerans revealed by next-generation sequencing.PLoS pathogens · 2009Article
- Custom design and analysis of high-density oligonucleotide bacterial tiling microarrays.PloS one · 2009Article
- A microarray based approach for the identification of common foodborne viruses.The open virology journal · 2009Article
- Microarray analyses of gene expression during the Tetrahymena thermophila life cycle.PloS one · 2009Article
- Novel genome polymorphisms in BCG vaccine strains and impact on efficacy.BMC genomics · 2008Article
- Review
- Complete genome sequence of Treponema pallidum ssp. pallidum strain SS14 determined with oligonucleotide arrays.BMC microbiology · 2008Article
- SNP-specific array-based allele-specific expression analysis.Genome research · 2008Article
- Microarray-based DNA resequencing using 3' blocked primers.Analytical biochemistry · 2008Article
- Applying rapid genome sequencing technologies to characterize pathogen genomes.Analytical chemistry · 2008Article
- In situ oligonucleotide synthesis on carbon materials: stable substrates for microarray fabrication.Nucleic acids research · 2008Article
- The transcription factor Mrr1p controls expression of the MDR1 efflux pump and mediates multidrug resistance in Candida albicans.PLoS pathogens · 2007Article
- Genome-wide expression and location analyses of the Candida albicans Tac1p regulon.Eukaryotic cell · 2007Article
- Protein binding microarrays for the characterization of DNA-protein interactions.Advances in biochemical engineering/biotechnology · 2007Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Light-directed synthesis of high-density microarrays is currently performed in the 3'-->5' direction due to constraints in existing synthesis chemistry. This results in the probes being unavailable for many common types of enzymatic modification. Arrays that are synthesized in the 5'-->3' direction could be utilized to perform parallel genotyping and resequencing directly on the array surface, dramatically increasing the throughput and reducing the cost relative to existing techniques. In this report we demonstrate the use of photoprotected phosphoramidite monomers for light-directed array synthesis in the 5'-->3' direction, using maskless array synthesis technology. These arrays have a dynamic range of >2.5 orders of magnitude, sensitivity below 1 pM and a coefficient of variance of <10% across the array surface. Arrays containing >150,000 probe sequences were hybridized to labeled mouse cRNA producing highly concordant data (average R(2) = 0.998). We have also shown that the 3' ends of array probes are available for sequence-specific primer extension and ligation reactions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.