ArticleNucleic acids research2003
Bypassing antibiotic selection: positive screening of genetically modified cells with an antigen-dependent proliferation switch.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- Synthetic control of implanted engineered liver tissue growth.Science advances · 2026Article
- Combination of Sleeping Beauty transposition and chemically induced dimerization selection for robust production of engineered cells.Nucleic acids research · 2012Article
- Bioluminescence assay for detecting cell surface membrane protein expression.Assay and drug development technologies · 2011Article
- An efficient vector system to modify cells genetically.PloS one · 2011Article
- Growth control of hybridoma cells with an artificially induced EpoR-gp130 heterodimer.Cytotechnology · 2006Article
- Human artificial chromosome vectors meet stem cells: new prospects for gene delivery.Stem cell reviews · 2006Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While antibiotic selection has been routinely used for the selection of genetically modified cells, administration of cytotoxic drugs often leads to deleterious effects not only to inert cells but also to transfected or transduced ones. In this study, we propose an Antigen-MEdiated Genetically modified cell Amplification (AMEGA) system employing antibody/receptor chimeras without antibiotic selection. Based on a rational design where the extracellular domains of dimeric erythropoietin receptor (EpoR) or gp130 were substituted with heterodimeric V(H)/V(L) regions of anti-hen egg lysozyme (HEL) antibody and EpoR D2 domains, the genes encoding the chimeras as well as a model transgene, enhanced green fluorescent protein (EGFP), were retrovirally infected into IL-3-dependent Ba/F3 cells followed by direct HEL selection in the absence of IL-3. After a single round of selection, EGFP-positive cells were selectively amplified, resulting in a population of almost 100% positive cells. The AMEGA without antibiotic selection will not harm normal cells, which will be especially useful for increasing the efficacy for stem cell-based gene therapy.
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Registered trials
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