ArticleThe Journal of cell biology2003
The Ran GTPase cycle is required for yeast nuclear pore complex assembly.
Article in The Journal of cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
- Structure, function and assembly of nuclear pore complexes.Nature reviews. Molecular cell biology · 2026Review
- How the chromatin landscape influences nuclear morphology.Frontiers in cell and developmental biology · 2025Review
- Article
- Review
- Structure, Maintenance, and Regulation of Nuclear Pore Complexes: The Gatekeepers of the Eukaryotic Genome.Cold Spring Harbor perspectives in biology · 2022Review
- Functional Analysis of an Essential GSP1/Ran Ortholog Gene,Journal of fungi (Basel, Switzerland) · 2021Article
- Nuclear envelope-vacuole contacts mitigate nuclear pore complex assembly stress.The Journal of cell biology · 2020Article
- Functional analysis of an essential Ran-binding protein gene, CpRbp1, from the chestnut blight fungus Cryphonectria parasitica using heterokaryon rescue.Scientific reports · 2020Article
- Article
- DNA damage triggers increased mobility of chromosomes in G1-phase cells.Molecular biology of the cell · 2019Article
- Live-Cell Imaging of mRNP-NPC Interactions in Budding Yeast.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Fantastic nuclear envelope herniations and where to find them.Biochemical Society transactions · 2018Review
- Chm7 and Heh1 collaborate to link nuclear pore complex quality control with nuclear envelope sealing.The EMBO journal · 2016Article
- Recent advances in understanding nuclear size and shape.Nucleus (Austin, Tex.) · 2016Review
- Nuclear transport factors: global regulation of mitosis.Current opinion in cell biology · 2015Review
- Nuclear pore basket proteins are tethered to the nuclear envelope and can regulate membrane curvature.Developmental cell · 2015Article
- The nuclear pore complex--structure and function at a glance.Journal of cell science · 2015Review
- Yeast importin-α (Srp1) performs distinct roles in the import of nuclear proteins and in targeting proteasomes to the nucleus.The Journal of biological chemistry · 2014Article
- Transportin acts to regulate mitotic assembly events by target binding rather than Ran sequestration.Molecular biology of the cell · 2014Article
- Defective nuclear import of Tpr in Progeria reflects the Ran sensitivity of large cargo transport.The Journal of cell biology · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Here, we report the first evidence that the Ran GTPase cycle is required for nuclear pore complex (NPC) assembly. Using a genetic approach, factors required for NPC assembly were identified in Saccharomyces cerevisiae. Four mutant complementation groups were characterized that correspond to respective mutations in genes encoding Ran (gsp1), and essential Ran regulatory factors Ran GTPase-activating protein (rna1), Ran guanine nucleotide exchange factor (prp20), and the RanGDP import factor (ntf2). All the mutants showed temperature-dependent mislocalization of green fluorescence protein (GFP)-tagged nucleoporins (nups) and the pore-membrane protein Pom152. A decrease in GFP fluorescence associated with the nuclear envelope was observed along with an increase in the diffuse, cytoplasmic signal with GFP foci. The defects did not affect the stability of existing NPCs, and nup mislocalization was dependent on de novo protein synthesis and continued cell growth. Electron microscopy analysis revealed striking membrane perturbations and the accumulation of vesicles in arrested mutants. Using both biochemical fractionation and immunoelectron microscopy methods, these vesicles were shown to contain nups. We propose a model wherein a Ran-mediated vesicular fusion step is required for NPC assembly into intact nuclear envelopes.
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Registered trials
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