ArticleInfection and immunity2003
Group A Streptococcus gene expression in humans and cynomolgus macaques with acute pharyngitis.
Article in Infection and immunity, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 83 citations in OpenAlex.
- An emm-type specific qPCR to track bacterial load during experimental human Streptococcus pyogenes pharyngitis.BMC infectious diseases · 2021Trial
- Streptococcus pyogenes carriage is associated with distinct in vivo transcriptional states but not carrier-specific genotypes.Cell reports · 2026Article
- The Slr protein of Streptococcus pyogenes is selectively expressed in vivo and is a target for protective antibodies.EMBO molecular medicine · 2026Article
- Development of a real-time multiple cross displacement amplification assay for rapid and highly sensitive detection of Streptococcus pyogenes.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025Article
- Analysis ofmBio · 2025Article
- TheJournal of bacteriology · 2025Article
- Development of the CRISPR-Cas12a-Based Biosensing System for Rapid, Ultrasensitive, and Highly Specific Detection ofACS omega · 2025Article
- Streptococcus pyogenes genes that promote pharyngitis in primates.JCI insight · 2020Article
- Strain-Dependent Effect of Capsule on Transmission and Persistence in an Infant Mouse Model of Group A Streptococcus Infection.Infection and immunity · 2020Article
- The detrimental impact of extracellular bacterial proteases on wound healing.International wound journal · 2017Review
- Unravelling pathogenetic mechanisms of epidemic lineages.Virulence · 2017Article
- Enhanced nasopharyngeal infection and shedding associated with an epidemic lineage of emm3 group A Streptococcus.Virulence · 2017Article
- A naturally occurring single amino acid replacement in multiple gene regulator of group A Streptococcus significantly increases virulence.The American journal of pathology · 2015Article
- The contribution of non-human primate models to the development of human vaccines.Discovery medicine · 2014Article
- Article
- Murine vaginal colonization model for investigating asymptomatic mucosal carriage of Streptococcus pyogenes.Infection and immunity · 2013Article
- Differential virulence gene expression of group A Streptococcus serotype M3 in response to co-culture with Moraxella catarrhalis.PloS one · 2013Article
- The non-human primate model of tuberculosis.Journal of medical primatology · 2012Review
- Surface interactome in Streptococcus pyogenes.Molecular & cellular proteomics : MCP · 2012Article
- Superantigenic activity of emm3 Streptococcus pyogenes is abrogated by a conserved, naturally occurring smeZ mutation.PloS one · 2012Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
Abstract
The molecular mechanisms used by group A Streptococcus (GAS) to survive on the host mucosal surface and cause acute pharyngitis are poorly understood. To provide new information about GAS host-pathogen interactions, we used real-time reverse transcription-PCR (RT-PCR) to analyze transcripts of 17 GAS genes in throat swab specimens taken from 18 pediatric patients with pharyngitis. The expression of known and putative virulence genes and regulatory genes (including genes in seven two-component regulatory systems) was studied. Several known and previously uncharacterized GAS virulence gene regulators were highly expressed compared to the constitutively expressed control gene proS. To examine in vivo gene transcription in a controlled setting, three cynomolgus macaques were infected with strain MGAS5005, an organism that is genetically representative of most serotype M1 strains recovered from pharyngitis and invasive disease episodes in North America and Western Europe. These three animals developed clinical signs and symptoms of GAS pharyngitis and seroconverted to several GAS extracellular proteins. Real-time RT-PCR analysis of throat swab material collected at intervals throughout a 12-day infection protocol indicated that expression profiles of a subset of GAS genes accurately reflected the profiles observed in the human pediatric patients. The results of our study demonstrate that analysis of in vivo GAS gene expression is feasible in throat swab specimens obtained from infected human and nonhuman primates. In addition, we conclude that the cynomolgus macaque is a useful nonhuman primate model for the study of molecular events contributing to acute pharyngitis caused by GAS.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.