ArticleGenome research2003
Chromosomal deletion formation system based on Tn5 double transposition: use for making minimal genomes and essential gene analysis.
Article in Genome research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Current Approaches for Genetic Manipulation ofBiotech (Basel (Switzerland)) · 2025Review
- Genome engineering on size reduction and complexity simplification: A review.Journal of advanced research · 2024Review
- Past, Present, and Future of Genome Modification inMicroorganisms · 2022Review
- Random genome reduction coupled with polyhydroxybutyrate biosynthesis to facilitate its accumulation inFrontiers in bioengineering and biotechnology · 2022Article
- Insights into the structure of Escherichia coli outer membrane as the target for engineering microbial cell factories.Microbial cell factories · 2021Review
- Advances and Perspectives for Genome Editing Tools ofFrontiers in microbiology · 2021Review
- An Update on Molecular Tools for Genetic Engineering of Actinomycetes-The Source of Important Antibiotics and Other Valuable Compounds.Antibiotics (Basel, Switzerland) · 2020Review
- Exploring the fitness benefits of genome reduction in Escherichia coli by a selection-driven approach.Scientific reports · 2020Article
- Coupling the recombineering to Cre-lox system enables simplified large-scale genome deletion in Lactobacillus casei.Microbial cell factories · 2018Article
- Assessing Tn5 and Sleeping Beauty for transpositional transgenesis by cytoplasmic injection into bovine and ovine zygotes.PloS one · 2017Article
- Article
- Emerging tools for synthetic genome design.Molecules and cells · 2013Review
- Random and cyclical deletion of large DNA segments in the genome of Pseudomonas putida.Environmental microbiology · 2012Article
- A further study on chromosome minimization by protoplast fusion in Aspergillus oryzae.Molecular genetics and genomics : MGG · 2012Article
- An improved particle bombardment for the generation of transgenic plants by direct immobilization of relleasable Tn5 transposases onto gold particles.Plant molecular biology · 2011Article
- Synthetic biology: From the first synthetic cell to see its current situation and future development.Chinese science bulletin = Kexue tongbao · 2011Review
- A trial of minimization of chromosome 7 in Aspergillus oryzae by multiple chromosomal deletions.Molecular genetics and genomics : MGG · 2010Article
- Characterization of YmgF, a 72-residue inner membrane protein that associates with the Escherichia coli cell division machinery.Journal of bacteriology · 2009Article
- Superpositioning of deletions promotes growth of Escherichia coli with a reduced genome.DNA research : an international journal for rapid publication of reports on genes and genomes · 2008Article
- Rapid and efficient construction of markerless deletions in the Escherichia coli genome.Nucleic acids research · 2008Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
In this communication, we describe the use of specialized transposons (Tn5 derivatives) to create deletions in the Escherichia coli K-12 chromosome. These transposons are essentially rearranged composite transposons that have been assembled to promote the use of the internal transposon ends, resulting in intramolecular transposition events. Two similar transposons were developed. The first deletion transposon was utilized to create a consecutive set of deletions in the E. coli chromosome. The deletion procedure has been repeated 20 serial times to reduce the genome an average of 200 kb (averaging 10 kb per deletion). The second deletion transposon contains a conditional origin of replication that allows deleted chromosomal DNA to be captured as a complementary plasmid. By plating cells on media that do not support plasmid replication, the deleted chromosomal material is lost and if it is essential, the cells do not survive. This methodology was used to analyze 15 chromosomal regions and more than 100 open reading frames (ORFs). This provides a robust technology for identifying essential and dispensable genes.
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Registered trials
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