ArticleAntimicrobial agents and chemotherapy2003
Engineering anthracycline biosynthesis toward angucyclines.
Article in Antimicrobial agents and chemotherapy, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 67 citations in OpenAlex.
- Heterologous Expression of Type II PKS Gene Cluster Leads to Diversified Angucyclines inMarine drugs · 2024Article
- Divergence of Classical and C-Ring-Cleaved Angucyclines: Elucidation of Early Tailoring Steps in Lugdunomycin and Thioangucycline Biosynthesis.ACS chemical biology · 2024Article
- Unravelling key enzymatic steps in C-ring cleavage during angucycline biosynthesis.Communications chemistry · 2023Article
- Overexpression of Global Regulator SCrp Leads to the Discovery of New Angucyclines inMarine drugs · 2023Article
- Rethinking Biosynthesis of Aclacinomycin A.Molecules (Basel, Switzerland) · 2023Review
- Genome mining of novel rubiginones from Streptomyces sp. CB02414 and characterization of the post-PKS modification steps in rubiginone biosynthesis.Microbial cell factories · 2021Article
- Biosynthesis of Diverse Type II Polyketide Core Structures inACS synthetic biology · 2021Article
- Ring D-Modified and Highly Reduced Angucyclinones From Marine Sediment-DerivedFrontiers in chemistry · 2021Article
- Functional and Structural Insights into a Novel Promiscuous Ketoreductase of the Lugdunomycin Biosynthetic Pathway.ACS chemical biology · 2020Article
- Early Steps in the Biosynthetic Pathway of Rishirilide B.Molecules (Basel, Switzerland) · 2020Article
- Assembling a plug-and-play production line for combinatorial biosynthesis of aromatic polyketides in Escherichia coli.PLoS biology · 2019Article
- PCR-Independent Method of Transformation-Associated Recombination Reveals the Cosmomycin Biosynthetic Gene Cluster in an Ocean Streptomycete.Journal of natural products · 2017Article
- Insights into Complex Oxidation during BE-7585A Biosynthesis: Structural Determination and Analysis of the Polyketide Monooxygenase BexE.ACS chemical biology · 2016Article
- Tailoring enzymes involved in the biosynthesis of angucyclines contain latent context-dependent catalytic activities.Chemistry & biology · 2012Article
- Angucyclines: Biosynthesis, mode-of-action, new natural products, and synthesis.Natural product reports · 2012Review
- Cyclization of aromatic polyketides from bacteria and fungi.Natural product reports · 2010Review
- Elucidation of oxygenation steps during oviedomycin biosynthesis and generation of derivatives with increased antitumor activity.Chembiochem : a European journal of chemical biology · 2009Article
- Isolation, characterization, and heterologous expression of the biosynthesis gene cluster for the antitumor anthracycline steffimycin.Applied and environmental microbiology · 2006Article
- Engineered biosynthesis of aklanonic acid analogues.Journal of the American Chemical Society · 2005Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The biosynthesis pathways of two anthracyclines, nogalamycin and aclacinomycin, were directed toward angucyclines by using an angucycline-specific cyclase, pgaF, isolated from a silent antibiotic biosynthesis gene cluster. Addition of pgaF to a gene cassette that harbored the early biosynthesis genes of nogalamycin resulted in the production of two known angucyclinone metabolites, rabelomycin and its precursor, UWM6. Substrate flexibility of pgaF was demonstrated by replacement of the nogalamycin minimal polyketide synthase genes in the gene cassette with the equivalent aclacinomycin genes together with aknE2 and aknF, which specify the unusual propionate starter unit in aclacinomycin biosynthesis. This modification led to the production of a novel angucyclinone, MM2002, in which the expected ethyl side chain was incorporated into the fourth ring.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.