ArticleJournal of biomolecular NMR2003
A sensitive and robust method for obtaining intermolecular NOEs between side chains in large protein complexes.
Article in Journal of biomolecular NMR, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Efficient and economic protein labeling for NMR in mammalian expression systems: Application to a preT-cell and T-cell receptor protein.Protein science : a publication of the Protein Society · 2024Article
- Experimental NOE, Chemical Shift, and Proline Isomerization Data Provide Detailed Insights into Amelotin Oligomerization.Journal of the American Chemical Society · 2023Article
- Optimized precursor to simplify assignment transfer between backbone resonances and stereospecifically labelled valine and leucine methyl groups: application to human Hsp90 N-terminal domain.Journal of biomolecular NMR · 2021Article
- Methyl group assignment using pseudocontact shifts with PARAssign.Journal of biomolecular NMR · 2017Article
- Highly efficient residue-selective labeling with isotope-labeled Ile, Leu, and Val using a new auxotrophic E. coli strain.Journal of biomolecular NMR · 2016Article
- Facilitating unambiguous NMR assignments and enabling higher probe density through selective labeling of all methyl containing amino acids.Journal of biomolecular NMR · 2016Article
- Scrambling free combinatorial labeling of alanine-β, isoleucine-δ1, leucine-proS and valine-proS methyl groups for the detection of long range NOEs.Journal of biomolecular NMR · 2015Article
- A 3D time-shared NOESY experiment designed to provide optimal resolution for accurate assignment of NMR distance restraints in large proteins.Journal of biomolecular NMR · 2014Article
- Selective methyl labeling of eukaryotic membrane proteins using cell-free expression.Journal of the American Chemical Society · 2014Article
- Independent valine and leucine isotope labeling in Escherichia coli protein overexpression systems.Journal of biomolecular NMR · 2013Article
- Perdeuteration and methyl-selective (1)H, (13)C-labeling by using a Kluyveromyces lactis expression system.Journal of biomolecular NMR · 2013Article
- Specific labeling and assignment strategies of valine methyl groups for NMR studies of high molecular weight proteins.Journal of biomolecular NMR · 2013Article
- High resolution methyl selective ¹³C-NMR of proteins in solution and solid state.Journal of biomolecular NMR · 2012Article
- Automated sequence- and stereo-specific assignment of methyl-labeled proteins by paramagnetic relaxation and methyl-methyl nuclear Overhauser enhancement spectroscopy.Journal of biomolecular NMR · 2011Article
- Observation of intermolecular interactions in large protein complexes by 2D-double difference nuclear Overhauser enhancement spectroscopy: application to the 44 kDa interferon-receptor complex.Journal of the American Chemical Society · 2011Article
- High-resolution methyl edited GFT NMR experiments for protein resonance assignments and structure determination.Journal of biomolecular NMR · 2010Article
- Intermolecular interactions in a 44 kDa interferon-receptor complex detected by asymmetric reverse-protonation and two-dimensional NOESY.Biochemistry · 2010Article
- Structural determination of biomolecular interfaces by nuclear magnetic resonance of proteins with reduced proton density.Journal of biomolecular NMR · 2010Article
- Site-specific labeling of proteins with NMR-active unnatural amino acids.Journal of biomolecular NMR · 2010Review
- Methyl groups as probes of supra-molecular structure, dynamics and function.Journal of biomolecular NMR · 2010Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
A method for measuring intermolecular NOEs in protein complexes based on asymmetric sample deuteration is described. (13)C/(1)H-I,L,V-methyl, U-(2)H labeled protein is produced using the biosynthetic precursors [gamma-(13)C]-alpha-ketobutyrate and [gamma,gamma'-(13)C(2)]-alpha-ketoisovalerate. The labeled protein is mixed with its unlabeled binding partner and a 3D (13)C-HMQC-NOESY is recorded, yielding unambiguous intermolecular aromatic/methyl NOEs. A simple synthesis of the biosynthetic precursors via reaction of diethyl oxalate with alkyl Grignard compounds is reported. The method is demonstrated for a 35 kDa heterodimeric protein complex dissolved in a CHAPS micelle. This approach will facilitate the solution structure determination of protein/protein, protein/ligand or protein/nucleic acid complexes.
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Registered trials
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