ArticleTransgenic research2003
Plastid transformation in Lesquerella fendleri, an oilseed Brassicacea.
Article in Transgenic research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 108 citations in OpenAlex.
- An efficient system for plastid transformation in an edible medicinal herb.Transgenic research · 2025Article
- Stable plastid transformation in kiwifruit (aBIOTECH · 2025Article
- Recent trends and advances in chloroplast engineering and transformation methods.Frontiers in plant science · 2025Review
- Multiple sgRNAs for one-step inactivation of the duplicated acetyl-coenzyme A carboxylase 2 (ACC2) genes in Brassica napus.Plant physiology · 2022Article
- Plastid Transformation: How Does it Work? Can it Be Applied to Crops? What Can it Offer?International journal of molecular sciences · 2020Review
- Investigating the In Vitro Regeneration Potential of Commercial Cultivars ofPlants (Basel, Switzerland) · 2019Article
- Construction of a species-specific vector for improved plastid transformation efficiency in3 Biotech · 2019Article
- Article
- Efficient Plastid Transformation in Arabidopsis.Plant physiology · 2017Article
- Stable plastid transformation inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2016Article
- Low frequency paternal transmission of plastid genes in Brassicaceae.Transgenic research · 2015Article
- Expression of the truncated tissue plasminogen activator (K2S) gene in tobacco chloroplast.Molecular biology reports · 2013Article
- Spectinomycin resistance mutations in the rrn16 gene are new plastid markers in Medicago sativa.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- Genetic engineering of radish: current achievements and future goals.Plant cell reports · 2011Review
- Spontaneous spectinomycin resistance mutations detected after biolistic transformation of Daucus carota L.Physiology and molecular biology of plants : an international journal of functional plant biology · 2011Article
- High efficiency plastid transformation in potato and regulation of transgene expression in leaves and tubers by alternative 5' and 3' regulatory sequences.Transgenic research · 2011Article
- Plant plastid engineering.Current genomics · 2010Article
- Plastid transformation in eggplant (Solanum melongena L.).Transgenic research · 2010Article
- High-level expression of active human alpha1-antitrypsin in transgenic tobacco chloroplasts.Transgenic research · 2009Article
- Genetic transformation of the sugar beet plastome.Transgenic research · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A plastid transformation protocol was developed for Lesquerella fendleri, a species with a high capacity for plant regeneration in tissue culture. Transformation vector pZS391B carried an aadA16gfp marker gene conferring streptomycin-spectinomycin resistance and green fluorescence under UV light. Biolistic transformation of 51 Lesquerella leaf samples, followed by spectinomycin selection, yielded two transplastomic clones. The AAD-GFP fusion protein, the marker gene product, was localized to chloroplasts by confocal laser microscopy. Fertile plants and seed progeny were obtained in line Lf-pZS391B-1. In the 51 samples a large number (108) of spontaneous mutants were identified. In five of the lines spectinomycin resistance was localized to a conserved stem structure by sequencing 16S rRNA genes. Success in L. fendleri, a wild oilseed species, extends plastid transformation beyond Arabidopsis thaliana in the Brassicaceae family.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.