ArticleTransgenic research2003
A self-excising Cre recombinase allows efficient recombination of multiple ectopic heterospecific lox sites in transgenic tobacco.
Article in Transgenic research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 49 citations in OpenAlex.
- Improving transient expression inFrontiers in plant science · 2024Article
- Effect of marker-free transgenic Chlamydomonas on the control of Aedes mosquito population and on plankton.Parasites & vectors · 2023Article
- Generation of marker-free transgenic hexaploid wheat via an Agrobacterium-mediated co-transformation strategy in commercial Chinese wheat varieties.Plant biotechnology journal · 2017Article
- Genetically encoded optical activation of DNA recombination in human cells.Chemical communications (Cambridge, England) · 2016Article
- The pollen- and embryo-specific Arabidopsis DLL promoter bears good potential for application in marker-free Cre/loxP self-excision strategy.Plant cell reports · 2015Article
- Efficient auto-excision of a selectable marker gene from transgenic citrus by combining the Cre/loxP system and ipt selection.Plant cell reports · 2013Article
- Targeted DNA excision in Arabidopsis by a re-engineered homing endonuclease.BMC biotechnology · 2012Article
- Recent advances in development of marker-free transgenic plants: regulation and biosafety concern.Journal of biosciences · 2012Review
- Article
- Transgene excision from wheat chromosomes by phage phiC31 integrase.Plant molecular biology · 2010Article
- Cre-mediated seed-specific transgene excision in tobacco.Plant molecular biology · 2010Article
- Expression of active Streptomyces phage phiC31 integrase in transgenic wheat plants.Plant cell reports · 2008Article
- Feasibility of the seed specific cruciferin C promoter in the self excision Cre/loxP strategy focused on generation of marker-free transgenic plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2008Article
- The maize Knotted1 gene is an effective positive selectable marker gene for Agrobacterium-mediated tobacco transformation.Plant cell reports · 2006Article
- Site-specific recombination in Zea mays.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- Inducible excision of selectable marker gene from transgenic plants by the cre/lox site-specific recombination system.Transgenic research · 2005Article
- Site-specific recombination in Arabidopsis plants promoted by the Integrase protein of coliphage HK022.Plant molecular biology · 2005Article
- PVX-Cre-mediated marker gene elimination from transgenic plants.Plant molecular biology · 2004Article
- The presence of a chromatin boundary appears to shield a transgene in tobacco from RNA silencing.The Plant cell · 2003Article
Corrections and comments
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2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To study the impact of different DNA configurations on the stability of transgene expression, a variant of the cre gene was developed. This variant allows for the highly efficient in planta removal of its own loxP-flanked coding sequence as well as other DNAs flanked by ectopic heterospecific lox sites, either lox511 or lox2272 or both, in trans. The plant intron-containing cre gene, creINT, was configured in such a way that self-excision generated an intact hygromycin resistance selectable marker gene. In this combination, all selected transformants showed highly efficient excision. Plants obtained showed no indication of any chimerism, indicating a cell autonomous nature of the hygromycin selection during transformation and regeneration. The highly efficient concomitant removal of wildtype and heterospecific lox site-flanked DNA demonstrated that upon retransformation with the self-excising creINT, sufficient amounts of Cre enzyme were produced prior to its removal. Plants obtained with creINT showed much less frequently the Cre-associated phenomenon of reduced fertility than plants obtained with a continuous presence of Cre recombinase. The creINT system has therefore advantages over systems with a continuously present Cre. The creINT system was successfully used for removal of two chromatin boundary elements from transgene cassettes in tobacco. Analysis of plants with and without boundary elements on the same chromosomal location will contribute to a better evaluation of the role of such elements in the regulation of transgene expression in plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.