ArticleTransgenic research2003
Tetracycline-inducible system for regulation of skeletal muscle-specific gene expression in transgenic mice.
Article in Transgenic research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Enrichment of the exocytosis protein STX4 in skeletal muscle remediates peripheral insulin resistance and alters mitochondrial dynamics via Drp1.Nature communications · 2022Article
- Changes in Skeletal Muscle PAK1 Levels Regulate Tissue Crosstalk to Impact Whole Body Glucose Homeostasis.Frontiers in endocrinology · 2022Article
- Activation of Crtc2/Creb1 in skeletal muscle enhances weight loss during intermittent fasting.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- CORP: Using transgenic mice to study skeletal muscle physiology.Journal of applied physiology (Bethesda, Md. : 1985) · 2020Review
- BDNF is a mediator of glycolytic fiber-type specification in mouse skeletal muscle.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- DOC2B promotes insulin sensitivity in mice via a novel KLC1-dependent mechanism in skeletal muscle.Diabetologia · 2019Article
- A novel tetracycline-responsive transgenic mouse strain for skeletal muscle-specific gene expression.Skeletal muscle · 2018Article
- RNA-seq and metabolomic analyses of Akt1-mediated muscle growth reveals regulation of regenerative pathways and changes in the muscle secretome.BMC genomics · 2017Article
- Genetically encoded optical activation of DNA recombination in human cells.Chemical communications (Cambridge, England) · 2016Article
- Targeted delivery of HGF to the skeletal muscle improves glucose homeostasis in diet-induced obese mice.Journal of physiology and biochemistry · 2015Article
- Metabolomic analysis of akt1-mediated muscle hypertrophy in models of diet-induced obesity and age-related fat accumulation.Journal of proteome research · 2015Article
- Akt1-mediated fast/glycolytic skeletal muscle growth attenuates renal damage in experimental kidney disease.Journal of the American Society of Nephrology : JASN · 2014Article
- Creb coactivators direct anabolic responses and enhance performance of skeletal muscle.The EMBO journal · 2014Article
- Constitutive expression of Yes-associated protein (Yap) in adult skeletal muscle fibres induces muscle atrophy and myopathy.PloS one · 2013Article
- Akt1-mediated skeletal muscle growth attenuates cardiac dysfunction and remodeling after experimental myocardial infarction.Circulation. Heart failure · 2012Article
- Myogenic Akt signaling attenuates muscular degeneration, promotes myofiber regeneration and improves muscle function in dystrophin-deficient mdx mice.Human molecular genetics · 2011Article
- Tetracycline-inducible promoter-based conditionally replicative adenoviruses for the control of viral replication.Cancer gene therapy · 2009Article
- A new mouse model for temporal- and tissue-specific control of extracellular superoxide dismutase.Genesis (New York, N.Y. : 2000) · 2009Article
- FGF21 is an Akt-regulated myokine.FEBS letters · 2008Article
- Fast/Glycolytic muscle fiber growth reduces fat mass and improves metabolic parameters in obese mice.Cell metabolism · 2008Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tightly regulated control of over-expression is often necessary to study one aspect or time point of gene function and, in transgenesis, may help to avoid lethal effects and complications caused by ubiquitous over-expression. We have utilized the benefits of an optimized tet-on system and a modified muscle creatine kinase (MCK) promoter to generate a skeletal muscle-specific, doxycycline (Dox) controlled over-expression system in transgenic mice. A DNA construct was generated in which the codon optimized reverse tetracycline transactivator (rtTA) was placed under control of a skeletal muscle-specific version of the mouse MCK promoter. Transgenic mice containing this construct expressed rtTA almost exclusively in skeletal muscles. These mice were crossed to a second transgenic line containing a bi-directional promoter centered on a tet responder element driving both a luciferase reporter gene and a tagged gene of interest; in this case the calpain inhibitor calpastatin. Compound hemizygous mice showed high level, Dox dependent muscle-specific luciferase activity often exceeding 10,000-fold over non-muscle tissues of the same mouse. Western and immunocytochemical analysis demonstrated similar Dox dependent muscle-specific induction of the tagged calpastatin protein. These findings demonstrate the effectiveness and flexibility of the tet-on system to provide a tightly regulated over-expression system in adult skeletal muscle. The MCKrtTA transgenic lines can be combined with other transgenic responder lines for skeletal muscle-specific over-expression of any target gene of interest.
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