ArticleTransgenic research2003
Transgenic expression of trypsin inhibitor CMe from barley in indica and japonica rice, confers resistance to the rice weevil Sitophilus oryzae.
Article in Transgenic research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 102 citations in OpenAlex.
- Spatially resolved transcriptomic analysis of the germinating barley grain.Nucleic acids research · 2023Article
- Overexpression of soybean trypsin inhibitor genes decreases defoliation by corn earworm (Frontiers in plant science · 2023Article
- FAR knockout significantly inhibits Chilo suppressalis survival and transgene expression of double-stranded FAR in rice exhibits strong pest resistance.Plant biotechnology journal · 2022Article
- Pleiotropic effects of recombinant protease inhibitors in plants.Frontiers in plant science · 2022Review
- Antixenosis, Antibiosis, and Potential Yield Compensatory Response in Barley Cultivars Exposed to Wheat Stem Sawfly (Hymenoptera: Cephidae) Under Field Conditions.Journal of insect science (Online) · 2020Article
- Plant Serine Protease Inhibitors: Biotechnology Application in Agriculture and Molecular Farming.International journal of molecular sciences · 2019Review
- Transgenic microRNA-14 rice shows high resistance to rice stem borer.Plant biotechnology journal · 2019Article
- Expression of two barley proteinase inhibitors in tomato promotes endogenous defensive response and enhances resistance to Tuta absoluta.BMC plant biology · 2018Article
- Arabidopsis Kunitz Trypsin Inhibitors in Defense Against Spider Mites.Frontiers in plant science · 2018Article
- Transcriptome Analysis of Ramie (Boehmeria nivea L. Gaud.) in Response to Ramie Moth (Cocytodes coerulea Guenée) Infestation.BioMed research international · 2016Article
- Gene pyramiding of peptidase inhibitors enhances plant resistance to the spider mite Tetranychus urticae.PloS one · 2012Article
- Article
- Effects of serine protease inhibitors on growth and development and digestive serine proteinases of the Sunn pest, Eurygaster integriceps.Journal of insect science (Online) · 2011Article
- Molecular cloning and expression of an alpha-amylase inhibitor from rye with potential for controlling insect pests.The protein journal · 2005Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Indica and japonica rice (Oryza sativa L.) plants were transformed by particle bombardment with the Itr1 gene encoding the barley trypsin inhibitor BTI-CMe, under the control of its own promoter that confers endosperm specificity, and the maize ubiquitin promoter. From 38 independent transgenic lines of indica (breeding line IR58) and 15 of the japonica (cv Senia) selected, 22 and 11, respectively, expressed the barley inhibitor at detectable levels. The transgene was correctly translated as indicated by western blot analysis with a level of expression in R3 seeds up to 0.31% (IR58) and 0.43% (Senia) of the total extracted protein. The functional integrity of BTI-CMe was confirmed by trypsin activity assays in liquid media and by activity staining gels, performed with seed extracts. The significant reduction of the survival rate of the rice weevil (Sitophilus oryzae, Coleoptera: Curculionidae) reared on homozygous transgenic indica and japonica rice seeds expressing the BTI-CMe, compared to non-transformed controls, and the decrease in the trypsin-like activity of insect crude midgut extracts, confirmed the utility of this proteinase inhibitor gene for the control of important storage pests.
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