ArticleTransgenic research2003
Elevation of transgene expression level by flanking matrix attachment regions (MAR) is promoter dependent: a study of the interactions of six promoters with the RB7 3' MAR.
Article in Transgenic research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 39 citations in OpenAlex.
- Lettuce-based production of an oral vaccine against porcine edema disease for the seed lot system.Plant biotechnology (Tokyo, Japan) · 2021Article
- Benefits of using genomic insulators flanking transgenes to increase expression and avoid positional effects.Scientific reports · 2019Article
- Review
- Chimeric 3' flanking regions strongly enhance gene expression in plants.Plant biotechnology journal · 2018Article
- The potential of transgenic green microalgae; a robust photobioreactor to produce recombinant therapeutic proteins.World journal of microbiology & biotechnology · 2014Review
- Analysis of gene sequences indicates that quantity not quality of chloroplast small HSPs improves thermotolerance in C4 and CAM plants.Plant cell reports · 2012Article
- Minimizing the unpredictability of transgene expression in plants: the role of genetic insulators.Plant cell reports · 2012Review
- Functional analysis of BnMAR element in transgenic tobacco plants.Molecular biology reports · 2011Article
- Design of gene constructs for transgenic maize.Methods in molecular biology (Clifton, N.J.) · 2009Review
- The influence of matrix attachment regions on transgene expression in Arabidopsis thaliana wild type and gene silencing mutants.Plant molecular biology · 2007Article
- Matrix attachment region from the chicken lysozyme locus reduces variability in transgene expression and confers copy number-dependence in transgenic rice plants.Plant cell reports · 2005Article
- Matrix attachment regions increase the efficiency and stability of RNA-mediated resistance to tomato spotted wilt virus in transgenic tobacco.Transgenic research · 2005Article
- Article
- The rb7 matrix attachment region increases the likelihood and magnitude of transgene expression in tobacco cells: a flow cytometric study.The Plant cell · 2005Article
- TM2, a novel strong matrix attachment region isolated from tobacco, increases transgene expression in transgenic rice calli and plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- Heritable transgene expression pattern imposed onto maize ubiquitin promoter by maize adh-1 matrix attachment regions: tissue and developmental specificity in maize transgenic plants.Plant cell reports · 2004Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have analyzed effects of a matrix attachment region (MAR) from the tobacco RB7 gene on transgene expression from six different promoters in stably transformed tobacco cell cultures. The presence of MARs flanking the transgene increased expression of constructs based on the constitutive CaMV 35S, NOS, and OCS promoters. Expression from an induced heat shock promoter was also increased and MARs did not cause expression in the absence of heat shock. There was also no effect of MARs on the pea ferredoxin promoter, which is not normally expressed in this cell line. Importantly, most transgenes flanked by RB7 MAR elements showed a large reduction in the number of low expressing GUS transformants relative to control constructs without MARs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.