ArticleJournal of the American Society for Mass Spectrometry2003
Top down characterization of secreted proteins from Mycobacterium tuberculosis by electron capture dissociation mass spectrometry.
Article in Journal of the American Society for Mass Spectrometry, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 77 citations in OpenAlex.
- The loss of the PDIM/PGL virulence lipids causes differential secretion of ESX-1 substrates inmSphere · 2024Article
- Fourier-transform ion cyclotron resonance mass spectrometry for characterizing proteoforms.Mass spectrometry reviews · 2022Review
- Novel Strategies to Address the Challenges in Top-Down Proteomics.Journal of the American Society for Mass Spectrometry · 2021Review
- Accurate Identification of Closely RelatedFrontiers in cellular and infection microbiology · 2021Article
- A Top-Down Proteomics Platform Coupling Serial Size Exclusion Chromatography and Fourier Transform Ion Cyclotron Resonance Mass Spectrometry.Analytical chemistry · 2019Article
- Top-Down Characterization of Proteins with Intact Disulfide Bonds Using Activated-Ion Electron Transfer Dissociation.Analytical chemistry · 2018Article
- Esx Systems and the Mycobacterial Cell Envelope: What's the Connection?Journal of bacteriology · 2017Review
- Scrutiny of Mycobacterium tuberculosis 19 kDa antigen proteoforms provides new insights in the lipoglycoprotein biogenesis paradigm.Scientific reports · 2017Article
- The glycosylated Rv1860 protein of Mycobacterium tuberculosis inhibits dendritic cell mediated TH1 and TH17 polarization of T cells and abrogates protective immunity conferred by BCG.PLoS pathogens · 2014Article
- Toward Understanding the Essence of Post-Translational Modifications for the Mycobacterium tuberculosis Immunoproteome.Frontiers in immunology · 2014Review
- Comprehensive analysis of protein modifications by top-down mass spectrometry.Circulation. Cardiovascular genetics · 2011Review
- Review
- Deciphering modifications in swine cardiac troponin I by top-down high-resolution tandem mass spectrometry.Journal of the American Society for Mass Spectrometry · 2010Article
- Top-down high-resolution mass spectrometry of cardiac myosin binding protein C revealed that truncation alters protein phosphorylation state.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Proteomic analysis of endodontic infections by liquid chromatography-tandem mass spectrometry.Oral microbiology and immunology · 2009Article
- Delineating Anopheles gambiae coactivator associated arginine methyltransferase 1 automethylation using top-down high resolution tandem mass spectrometry.Protein science : a publication of the Protein Society · 2009Article
- An integrated top-down and bottom-up strategy for broadly characterizing protein isoforms and modifications.Journal of proteome research · 2009Article
- Electron transfer dissociation of doubly sodiated glycerophosphocholine lipids.Journal of the American Society for Mass Spectrometry · 2007Article
- A pulsed triple ionization source for sequential ion/ion reactions in an electrodynamic ion trap.Journal of the American Society for Mass Spectrometry · 2007Article
- The role of conformation on electron capture dissociation of ubiquitin.Journal of the American Society for Mass Spectrometry · 2006Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Secreted proteins of Mycobacterium tuberculosis are implicated in its disease pathogenesis and so are considered as potential diagnostic and vaccine candidates. The search for these has been slow, even though the entire genome sequence of M. tuberculosis is now available; of the 620 protein spots resolved by 2-D gel electrophoresis, 114 secreted proteins have been identified, but for only 13 has the primary structure been partly characterized. For comparison, in this top down mass spectrometry (MS) approach the secreted proteins were precipitated from cell culture filtrate, resuspended, and examined directly by electrospray ionization (ESI) Fourier transform MS. The ESI spectra of three precipitates showed 93, 535, and 369 molecular weight (M(r)) values, for a total of 689 different values. However, only approximately 10% of these values matched (+/-1 Da) the DNA predicted M(r) values, but these identifications were unreliable. Of nine molecular ions characterized by MS/MS, only one protein match was confirmed, and its isotopic molecular ions were overlapped by those of another protein. MS/MS identified a total of ten proteins by sequence tag search, of which three were unidentified previously. The low success of M(r) matching was due to unusually extensive posttranslational modifications, including loss of a signal sequence, loss of the N-terminal residue, proteolytic degradation, oxidation, and glycosylation. Although in eubacteria the latter is relatively rare, a 9 kDa protein showed 7 hexose attachments and two 20 kDa proteins each had 20 attachments. For MS/MS, electron capture dissociation was especially effective.
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