ArticleThe Journal of biological chemistry2003
Enhancer-dependent 5'-splice site control of fruitless pre-mRNA splicing.
Article in The Journal of biological chemistry, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Article
- Review
- Characterization of the doublesex gene within the Culex pipiens complex suggests regulatory plasticity at the base of the mosquito sex determination cascade.BMC evolutionary biology · 2015Article
- Neuroethology of male courtship in Drosophila: from the gene to behavior.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2014Review
- Kinetic analysis of in vitro pre-mRNA splicing in HeLa nuclear extract.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Article
- An exon splice enhancer primes IGF2:IGF2R binding site structure and function evolution.Science (New York, N.Y.) · 2012Article
- Molecular design of a splicing switch responsive to the RNA binding protein Tra2β.Nucleic acids research · 2011Article
- Age, but not experience, affects courtship gene expression in male Drosophila melanogaster.PloS one · 2009Article
- Spliceosome assembly pathways for different types of alternative splicing converge during commitment to splice site pairing in the A complex.Molecular and cellular biology · 2009Article
- Dynamic regulation of alternative splicing by silencers that modulate 5' splice site competition.Cell · 2008Article
- Gene discovery in an invasive tephritid model pest species, the Mediterranean fruit fly, Ceratitis capitata.BMC genomics · 2008Article
- Multisite and bidirectional exonic splicing enhancer in CD44 alternative exon v3.RNA (New York, N.Y.) · 2007Article
- The doublesex splicing enhancer components Tra2 and Rbp1 also repress splicing through an intronic silencer.Molecular and cellular biology · 2007Article
- Activation of alpha-tropomyosin exon 2 is regulated by the SR protein 9G8 and heterogeneous nuclear ribonucleoproteins H and F.Molecular and cellular biology · 2006Article
- The splicing regulators Tra and Tra2 are unusually potent activators of pre-mRNA splicing.Nucleic acids research · 2006Article
- The architecture of pre-mRNAs affects mechanisms of splice-site pairing.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Identification of sex-specific transcripts of the Anopheles gambiae doublesex gene.The Journal of experimental biology · 2005Article
- Mer1p is a modular splicing factor whose function depends on the conserved U2 snRNP protein Snu17p.Nucleic acids research · 2004Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The Drosophila fruitless (fru) gene encodes a transcription factor that essentially regulates all aspects of male courtship behavior. The use of alternative 5'-splice sites generates fru isoforms that determine gender-appropriate sexual behaviors. Alternative splicing of fru is regulated by TRA and TRA2 and depends on an exonic splicing enhancer (fruRE) consisting of three 13-nucleotide repeat elements, nearly identical to those that regulate alternative sex-specific 3'-splice site choice in the doublesex (dsx) gene. dsx has provided a useful model system to investigate the mechanisms of enhancer-dependent 3'-splice site choice. However, little is known about enhancer-dependent regulation of alternative 5'-splice sites. The mechanisms of this process were investigated using an in vitro system in which recombinant TRA/TRA2 could activate the female-specific 5'-splice site of fru. Mutational analysis demonstrated that one 13-nucleotide repeat element within the fruRE is required and sufficient to activate the regulated female-specific splice site. As was established for dsx, the fruRE can be replaced by a short element encompassing tandem 13-nucleotide repeat elements, by heterologous splicing enhancers, and by artificially tethering a splicing activator to the pre-mRNA. Complementation experiments showed that Ser/Arg-rich proteins facilitate enhancer-dependent 5'-splice site activation. We conclude that splicing enhancers function similarly in activating regulated 5'- and 3'-splice sites. These results suggest that exonic splicing enhancers recruit multiple spliceosomal components required for the initial recognition of 5'- and 3'-splice sites.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.