ArticleReproductive biology and endocrinology : RB&E2003
Analysis and characterization of differential gene expression during rapid trophoblastic elongation in the pig using suppression subtractive hybridization.
Article in Reproductive biology and endocrinology : RB&E, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 80 citations in OpenAlex.
- Article
- Porcine endometrial heat shock proteins are differentially influenced by pregnancy status, heat stress, and altrenogest supplementation during the peri-implantation period.Journal of animal science · 2022Article
- Global analysis of differential gene expression within the porcine conceptus transcriptome as it transitions through spherical, ovoid, and tubular morphologies during the initiation of elongation.Molecular reproduction and development · 2022Article
- Association ofAnimals : an open access journal from MDPI · 2021Article
- Integrating LCM-Based Spatio-Temporal Transcriptomics Uncovers Conceptus and Endometrial Luminal Epithelium Communication that Coordinates the Conceptus Attachment in Pigs.International journal of molecular sciences · 2021Article
- Effects of 17β-estradiol, Interleukin-1β, and Human Chorionic Gonadotropin on Activity and mRNA Expression of Plasminogen Activators in Porcine Endometrial Cells.Development & reproduction · 2018Article
- Higher Expression of Toll-Like Receptors 2 and 4 in Blood Cells of Keratoconus Patiens.Scientific reports · 2017Article
- Within-litter variation in birth weight: impact of nutritional status in the sow.Journal of Zhejiang University. Science. B · 2015Review
- Endometrial gene expression profiling in pregnant Meishan and Yorkshire pigs on day 12 of gestation.BMC genomics · 2014Article
- Cytokines from the pig conceptus: roles in conceptus development in pigs.Journal of animal science and biotechnology · 2014Review
- Interdisciplinary collaborative team for blastocyst implantation research: inception and perspectives.American journal of reproductive immunology (New York, N.Y. : 1989) · 2014Article
- Interaction of the conceptus and endometrium to establish pregnancy in mammals: role of interleukin 1β.Cell and tissue research · 2012Review
- Microarray Analysis of Gene Expression in the Uterine Endometrium during the Implantation Period in Pigs.Asian-Australasian journal of animal sciences · 2012Article
- Microarray profiling for differential gene expression in PMSG-hCG stimulated preovulatory ovarian follicles of Chinese Taihu and Large White sows.BMC genomics · 2011Article
- Endometrial caspase 1 and interleukin-18 expression during the estrous cycle and peri-implantation period of porcine pregnancy and response to early exogenous estrogen administration.Reproductive biology and endocrinology : RB&E · 2010Article
- The involvement of proline-rich 15 in early conceptus development in sheep.Biology of reproduction · 2009Article
- Expression of toll-like receptor 9 in peripheral blood mononuclear cells from patients with different hepatitis B and C viral loads.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2009Article
- HA117 gene increased the multidrug resistance of K562 cells in vitro: an investigation to the function of a novel gene related to drug resistance.Journal of experimental & clinical cancer research : CR · 2009Article
- Advances in swine transcriptomics.International journal of biological sciences · 2007Review
- The Early Stages of Implantation and Placentation in the Pig.Advances in anatomy, embryology, and cell biologyArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During late peri-implantation development, porcine conceptuses undergo a rapid (2-3 hrs) morphological transformation from a 10 mm sphere to a thin filamentous form greater than 150 mm in length. Elongation of the conceptus is important for establishing adequate placental surface area needed for embryo and fetal survival throughout gestation. Genes involved with triggering this unique transition in conceptus development are not well defined. Objective of the present study was to utilize suppression subtractive hybridization (SSH) to characterize the change in gene expression during conceptus transformation from spherical (8-9 mm) to tubular (15-40 mm) to early filamentous (>150 mm) morphology. Spherical, tubular, and filamentous conceptuses were collected from pregnant gilts and subjected to SSH. Forward and reverse subtractions were performed to identify candidate genes differentially expressed during spherical to tubular and tubular to filamentous transition. A total of 384 transcripts were differentially screened to ensure unique expression. Of the transcripts screened, sequences were obtained for 142 that were confirmed to be differentially expressed among the various morphologies. Gene expression profiles during rapid trophoblastic elongation were generated for selected mRNAs using quantitative real-time PCR. During the transition from tubular to early filamentous conceptuses, s-adenosylhomocysteine hydrolase and heat shock cognate 70 kDa expression were significantly enhanced. A novel unknown gene was isolated and shown to be significantly up-regulated at the onset of rapid trophoblastic elongation and further enhanced in filamentous conceptuses.
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