ArticlePlant physiology2003
Proteome analysis. Novel proteins identified at the peribacteroid membrane from Lotus japonicus root nodules.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
59 citing papers in PubMed, 199 citations in OpenAlex.
- ESN3, a nodule-specific small peptide essential for symbiotic nitrogen fixation in Medicago truncatula.The New phytologist · 2026Article
- Symbiotic peptides modulate rhizobial physiology without terminal differentiation.Science advances · 2026Article
- Symbiosome functionality in Medicago truncatula nodules requires continuous clearing of pectins from the symbiosome space.Nature communications · 2025Article
- Review
- Mapping the molecular landscape of Lotus japonicus nodule organogenesis through spatiotemporal transcriptomics.Nature communications · 2024Article
- Malate Transport and Metabolism in Nitrogen-Fixing Legume Nodules.Molecules (Basel, Switzerland) · 2021Review
- Plant-Microbe Interaction: Aboveground to Belowground, from the Good to the Bad.International journal of molecular sciences · 2021Review
- PHO1 family members transport phosphate from infected nodule cells to bacteroids in Medicago truncatula.Plant physiology · 2021Article
- Celebrating 20 Years of Genetic Discoveries in Legume Nodulation and Symbiotic Nitrogen Fixation.The Plant cell · 2020Review
- Understanding transport processes in lichen,F1000Research · 2020Review
- An Alkane Sulfonate Monooxygenase Is Required for Symbiotic Nitrogen Fixation byApplied and environmental microbiology · 2019Article
- Article
- Sulfate is transported at significant rates through the symbiosome membrane and is crucial for nitrogenase biosynthesis.Plant, cell & environment · 2019Article
- Involvement of Glutaredoxin and Thioredoxin Systems in the Nitrogen-Fixing Symbiosis between Legumes and Rhizobia.Antioxidants (Basel, Switzerland) · 2018Review
- Proteomic analysis dissects the impact of nodulation and biological nitrogen fixation on Vicia faba root nodule physiology.Plant molecular biology · 2018Article
- Quantitative proteomics of a BThe New phytologist · 2018Article
- Sulfur Transport and Metabolism in Legume Root Nodules.Frontiers in plant science · 2018Review
- The Nitrate Transporter Family Protein LjNPF8.6 Controls the N-Fixing Nodule Activity.Plant physiology · 2017Article
- Article
- The Symbiosome: Legume and Rhizobia Co-evolution toward a Nitrogen-Fixing Organelle?Frontiers in plant science · 2017Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The peribacteroid membrane (PBM) forms the structural and functional interface between the legume plant and the rhizobia. The model legume Lotus japonicus was chosen to study the proteins present at the PBM by proteome analysis. PBM was purified from root nodules by an aqueous polymer two-phase system. Extracted proteins were subjected to a global trypsin digest. The peptides were separated by nanoscale liquid chromatography and analyzed by tandem mass spectrometry. Searching the nonredundant protein database and the green plant expressed sequence tag database using the tandem mass spectrometry data identified approximately 94 proteins, a number far exceeding the number of proteins reported for the PBM hitherto. In particular, a number of membrane proteins like transporters for sugars and sulfate; endomembrane-associated proteins such as GTP-binding proteins and vesicle receptors; and proteins involved in signaling, for example, receptor kinases, calmodulin, 14-3-3 proteins, and pathogen response-related proteins, including a so-called HIR protein, were detected. Several ATPases and aquaporins were present, indicating a more complex situation than previously thought. In addition, the unexpected presence of a number of proteins known to be located in other compartments was observed. Two characteristic protein complexes obtained from native gel electrophoresis of total PBM proteins were also analyzed. Together, the results identified specific proteins at the PBM involved in important physiological processes and localized proteins known from nodule-specific expressed sequence tag databases to the PBM.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.