ArticleJournal of bacteriology2003
Architecture of a fur binding site: a comparative analysis.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed, 94 citations in OpenAlex.
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- Inferred regulons are consistent with regulator binding sequences in E. coli.PLoS computational biology · 2024Article
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- Unusual Relationship between Iron Deprivation and Organophosphate Hydrolase Expression.Applied and environmental microbiology · 2023Article
- T6SS secretes an LPS-binding effector to recruit OMVs for exploitative competition and horizontal gene transfer.The ISME journal · 2022Article
- Regulatory involvement of the PerR and SloR metalloregulators in theJournal of bacteriology · 2021Article
- Biochemical characterization of ferric uptake regulator (Fur) from Aliivibrio salmonicida. Mapping the DNA sequence specificity through binding studies and structural modelling.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2020Article
- Two novel proteins, TtpB2 and TtpD2, are essential for iron transport in the TonB2 system of Vibrio vulnificus.MicrobiologyOpen · 2020Article
- Characterization of temperature-dependent hemin uptake receptors HupA and HvtA in Vibrio vulnificus.MicrobiologyOpen · 2019Article
- Dissociation between Iron and Heme Biosyntheses Is Largely Accountable for Respiration Defects ofApplied and environmental microbiology · 2018Article
- OProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Transcriptional and posttranscriptional regulation of Shigella shuT in response to host-associated iron availability and temperature.MicrobiologyOpen · 2017Article
- The Role of the Regulator Fur in Gene Regulation and Virulence ofFrontiers in cellular and infection microbiology · 2017Article
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- Mechanistic insights into metal ion activation and operator recognition by the ferric uptake regulator.Nature communications · 2015Article
- Fur is the master regulator of the extraintestinal pathogenic Escherichia coli response to serum.mBio · 2014Article
- The iron stimulon and fur regulon of Geobacter sulfurreducens and their role in energy metabolism.Applied and environmental microbiology · 2014Article
- Identification of a core sequence for the binding of BosR to the rpoS promoter region in Borrelia burgdorferi.Microbiology (Reading, England) · 2014Article
- The FurA regulon in Anabaena sp. PCC 7120: in silico prediction and experimental validation of novel target genes.Nucleic acids research · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Fur is an iron-binding transcriptional repressor that recognizes a 19-bp consensus site of the sequence 5'-GATAATGATAATCATTATC-3'. This site can be defined as three adjacent hexamers of the sequence 5'-GATAAT-3', with the third being slightly imperfect (an F-F-F configuration), or as two hexamers in the forward orientation separated by one base pair from a third hexamer in the reverse orientation (an F-F-x-R configuration). Although Fur can bind synthetic DNA sequences containing the F-F-F arrangement, most natural binding sites are variations of the F-F-x-R arrangement. The studies presented here compared the ability of Fur to recognize synthetic DNA sequences containing two to four adjacent hexamers with binding to sequences containing variations of the F-F-x-R arrangement (including natural operator sequences from the entS and fepB promoter regions of Escherichia coli). Gel retardation assays showed that the F-F-x-R architecture was necessary for high-affinity Fur-DNA interactions and that contiguous hexamers were not recognized as effectively. In addition, the stoichiometry of Fur at each binding site was determined, showing that Fur interacted with its minimal 19-bp binding site as two overlapping dimers. These data confirm the proposed overlapping-dimer binding model, where the unit of interaction with a single Fur dimer is two inverted hexamers separated by a C:G base pair, with two overlapping units comprising the 19-bp consensus binding site required for the high-affinity interaction with two Fur dimers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.