ArticleMolecular and cellular biology2003
Synthesis of signals for de novo DNA methylation in Neurospora crassa.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 61 citations in OpenAlex.
- RID is required for both repeat-induced point mutation and nucleation of a novel transitional heterochromatic state for euchromatic repeats.Nucleic acids research · 2025Article
- DNA methylation-dependent epigenetic regulation ofaBIOTECH · 2023Article
- Identification of Putative Virulence Genes by DNA Methylation Studies in the Cereal PathogenCells · 2021Article
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- Comparative analysis of genome-wide DNA methylation inEpigenetics · 2020Article
- Writing and Reading Histone H3 Lysine 9 Methylation inFrontiers in plant science · 2020Review
- DNA Methylation: Shared and Divergent Features across Eukaryotes.Trends in genetics : TIG · 2019Review
- Evolution and genome architecture in fungal plant pathogens.Nature reviews. Microbiology · 2017Review
- Induction of H3K9me3 and DNA methylation by tethered heterochromatin factors inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Dual chromatin recognition by the histone deacetylase complex HCHC is required for proper DNA methylation in Neurospora crassa.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Non-exhaustive DNA methylation-mediated transposon silencing in the black truffle genome, a complex fungal genome with massive repeat element content.Genome biology · 2014Article
- Identification of genomic features in environmentally induced epigenetic transgenerational inherited sperm epimutations.PloS one · 2014Article
- Neurospora crassa, a model system for epigenetics research.Cold Spring Harbor perspectives in biology · 2013Review
- Ing1 functions in DNA demethylation by directing Gadd45a to H3K4me3.Genes & development · 2013Article
- An 11bp region with stem formation potential is essential for de novo DNA methylation of the RPS element.PloS one · 2013Article
- Heterochromatin is required for normal distribution of Neurospora crassa CenH3.Molecular and cellular biology · 2011Article
- DNA methylation and normal chromosome behavior in Neurospora depend on five components of a histone methyltransferase complex, DCDC.PLoS genetics · 2010Article
- Confining euchromatin/heterochromatin territory: jumonji crosses the line.Genes & development · 2010Review
- Identification of DIM-7, a protein required to target the DIM-5 H3 methyltransferase to chromatin.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- The DMM complex prevents spreading of DNA methylation from transposons to nearby genes in Neurospora crassa.Genes & development · 2010Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Most 5-methylcytosine in Neurospora crassa occurs in A:T-rich sequences high in TpA dinucleotides, hallmarks of repeat-induced point mutation. To investigate how such sequences induce methylation, we developed a sensitive in vivo system. Tests of various 25- to 100-bp synthetic DNA sequences revealed that both T and A residues were required on a given strand to induce appreciable methylation. Segments composed of (TAAA)(n) or (TTAA)(n) were the most potent signals; 25-mers induced robust methylation at the special test site, and a 75-mer induced methylation elsewhere. G:C base pairs inhibited methylation, and cytosines 5' of ApT dinucleotides were particularly inhibitory. Weak signals could be strengthened by extending their lengths. A:T tracts as short as two were found to cooperate to induce methylation. Distamycin, which, like the AT-hook DNA binding motif found in proteins such as mammalian HMG-I, binds to the minor groove of A:T-rich sequences, suppressed DNA methylation and gene silencing. We also found a correlation between the strength of methylation signals and their binding to an AT-hook protein (HMG-I) and to activities in a Neurospora extract. We propose that de novo DNA methylation in Neurospora cells is triggered by cooperative recognition of the minor groove of multiple short A:T tracts. Similarities between sequences subjected to repeat-induced point mutation in Neurospora crassa and A:T-rich repeated sequences in heterochromatin in other organisms suggest that related mechanisms control silent chromatin in fungi, plants, and animals.
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