ArticleDiabetologia2003
C-peptide stimulates Na+,K+-ATPase activity via PKC alpha in rat medullary thick ascending limb.
Article in Diabetologia, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 81 citations in OpenAlex.
- Salt-Sensitivity of Blood Pressure and Insulin Resistance.Frontiers in physiology · 2021Review
- Biological Activity of c-Peptide in Microvascular Complications of Type 1 Diabetes-Time for Translational Studies or Back to the Basics?International journal of molecular sciences · 2020Review
- Association between a novel G94A single nucleotide polymorphism inJournal of research in medical sciences : the official journal of Isfahan University of Medical Sciences · 2019Article
- Targeting orphan G protein-coupled receptors for the treatment of diabetes and its complications: C-peptide and GPR146.Journal of internal medicine · 2017Review
- PKC-α contributes to high NaCl-induced activation of NFAT5 (TonEBP/OREBP) through MAPK ERK1/2.American journal of physiology. Renal physiology · 2015Article
- Angiotensin II-induced hypertension increases plasma membrane Na pump activity by enhancing Na entry in rat thick ascending limbs.American journal of physiology. Renal physiology · 2013Article
- Review
- NADPH oxidase and PKC contribute to increased Na transport by the thick ascending limb during type 1 diabetes.Hypertension (Dallas, Tex. : 1979) · 2012Article
- Insulin resistance, obesity, hypertension, and renal sodium transport.International journal of hypertension · 2011Article
- Article
- C-Peptide and its intracellular signaling.The review of diabetic studies : RDS · 2009Article
- Angiotensin II-dependent hypertension increases Na transport-related oxygen consumption by the thick ascending limb.Hypertension (Dallas, Tex. : 1979) · 2008Article
- Proteinase-activated receptor 2 stimulates Na,K-ATPase and sodium reabsorption in native kidney epithelium.The Journal of biological chemistry · 2008Article
- Renal and vascular benefits of C-peptide: Molecular mechanisms of C-peptide action.Biologics : targets & therapy · 2008Article
- Proinsulin C-peptide: friend or foe in the development of diabetes-associated complications?Vascular health and risk management · 2008Review
- C-Peptide effects on renal physiology and diabetes.Experimental diabetes research · 2008Review
- C-peptide and its C-terminal fragments improve erythrocyte deformability in type 1 diabetes patients.Experimental diabetes research · 2008Article
- Intracellular signalling by C-peptide.Experimental diabetes research · 2008Review
- C-peptide is a bioactive peptide.Diabetologia · 2007Article
- C-peptide stimulates ERK1/2 and JNK MAP kinases via activation of protein kinase C in human renal tubular cells.Diabetologia · 2005Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aims/hypothesisC-peptide, the cleavage product of proinsulin processing exerts physiological effects including stimulation of Na(+),K(+)-ATPase in erythrocytes and renal proximal tubules. This study was undertaken to assess the physiological effects of connecting peptide on Na(+),K(+)-ATPase activity in the medullary thick ascending limb of Henle's loop.
methodsNa(+),K(+)-ATPase activity was measured as the ouabain-sensitive generation of (32)Pi from gamma[(32)P]-ATP and (86)Rb uptake on isolated rat medullary thick ascending limbs. The cell-surface expression of Na(+),K(+)-ATPase was evaluated by Western blotting of biotinylated proteins, and its phosphorylation amount was measured by autoradiography. The membrane-associated fraction of protein kinase C isoforms was evaluated by Western blotting.
resultsRat connecting peptide concentration-dependently stimulated Na(+),K(+)-ATPase activity with a threshold at 10(-9) mol/l and a maximal effect at 10(-7) mol/l. C-peptide (10(-7) mol/l) already stimulates Na(+),K(+)-ATPase activity after 5 min with a plateau from 15 to 60 min. C-peptide (10(-7) mol/l) stimulated Na(+),K(+)-ATPase activity and (86)Rb uptake to the same extent, but did not alter Na(+),K(+)-ATPase cell surface expression. The stimulation of Na(+),K(+)-ATPase activity was associated with an increase in Na(+),K(+)-ATPase alpha-subunit phosphorylation and both effects were abolished by a specific protein kinase C inhibitor. Furthermore, connecting peptide induced selective membrane translocation of PKC-alpha. CONCLUSION/
interpretationThis study provides evidence that in rat medullary thick ascending limb, C-peptide stimulates Na(+),K(+)-ATPase activity within a physiological concentration range. This effect is due to an increase in Na(+),K(+)-ATPase turnover rate that is most likely mediated by protein kinase C-alpha phosphorylation of the Na(+),K(+)-ATPase alpha-subunit, suggesting that C-peptide could control Na(+) reabsorption during non-fasting periods.
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