ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2003
Use of induced sputum for the diagnosis of influenza and infections in asthma: a comparison of diagnostic techniques.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 5 of them syntheses that pooled it.
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Who cites it
24 citing papers in PubMed, 5 syntheses or guidelines pooled it, 48 citations in OpenAlex.
- Diagnostic accuracy of point-of-care tests for acute respiratory infection: a systematic review of reviews.Health technology assessment (Winchester, England) · 2026Pooled it
- Prevalence of respiratory viruses in stable and acute asthma: a systematic review and meta-analysis.European respiratory review : an official journal of the European Respiratory Society · 2026Pooled it
- Underascertainment of Respiratory Syncytial Virus Infection in Adults Due to Diagnostic Testing Limitations: A Systematic Literature Review and Meta-analysis.The Journal of infectious diseases · 2023Pooled it
- Pooled it
- Seasonal influenza in adults and children--diagnosis, treatment, chemoprophylaxis, and institutional outbreak management: clinical practice guidelines of the Infectious Diseases Society of America.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2009Guideline
- Navigating an evolving microbial landscape: emerging antimicrobial resistance trends and precision stewardship in Tianjin tertiary hospitals (2021-2023).Frontiers in cellular and infection microbiology · 2025Article
- Pregnancy enhances antiviral immunity independent of type I IFN but dependent on IL-17-producing γδScience advances · 2024Article
- Article
- Boosting nitric oxide in stress and respiratory infection: Potential relevance for asthma and COVID-19.Brain, behavior, & immunity - health · 2021Review
- Granulocyte macrophage colony-stimulating factor has come of age: From a vaccine adjuvant to antiviral immunotherapy.Cytokine & growth factor reviews · 2021Review
- Picornavirus-Induced Airway Mucosa Immune Profile in Asymptomatic Neonates.The Journal of infectious diseases · 2016Article
- Infection in severe asthma exacerbations and critical asthma syndrome.Clinical reviews in allergy & immunology · 2015Review
- Respiratory syncytial virus infection modifies and accelerates pulmonary disease via DC activation and migration.Journal of leukocyte biology · 2013Article
- Yield of sputum for viral detection by reverse transcriptase PCR in adults hospitalized with respiratory illness.Journal of clinical microbiology · 2012Article
- Repeated virus identification in the airways of patients with mild and severe asthma during prospective follow-up.Allergy · 2011Article
- Respiratory syncytial virus and human rhinoviruses are the major causes of severe lower respiratory tract infections in Kuwait.Journal of medical virology · 2010Article
- Article
- Antimicrobial strategies: an option to treat allergy?Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2007Review
- New treatment regimes for virus-induced exacerbations of asthma.Pulmonary pharmacology & therapeutics · 2006Review
- Improved diagnosis of the etiology of community-acquired pneumonia with real-time polymerase chain reaction.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2005Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInfluenza (Flu) and respiratory syncytial virus (RSV) are important viral pathogens that cause lower respiratory tract infections and severe exacerbations of asthma. Molecular biological techniques are permitting a rapid and accurate diagnosis of infections caused by respiratory pathogens, and have typically been applied to upper respiratory samples. Sputum induction provides an opportunity to directly sample secretions from the lower respiratory tract. OBJECTIVES/STUDY
designTo determine the role of induced sputum reverse-transcription polymerase chain reaction (RT-PCR) in the detection of respiratory pathogens and compare this with detection using serology and immunofluorescent antigen (IFA) testing, we recruited 49 adults from emergency room with exacerbations of asthma. After a medical assessment and spirometry, sputum was induced using ultrasonically nebulised normal saline. Sputum was assayed using IFA and RT-PCR for flu and RSV. Flu serology was performed acutely and at convalescence, 4-5 weeks later.
resultsInfluenza A or B was detected in 24% of the samples by PCR, significantly more than the nine cases detected using serology and the one case using IFA (P<0.05). RSV was detected in 37% of samples using PCR and 20% by IFA (P<0.05).
conclusionThe combination of induced sputum and RT-PCR provides a useful means of detecting respiratory infection. The technique is safe in both adults and children, and RT-PCR is more sensitive than conventional serology and IFA. The improved sensitivity of induced sputum RT-PCR also permits a more rapid diagnosis and the opportunity of early administration of effective treatments.
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