ArticlePharmaceutical research2003
Ligand-specific targeting of microspheres to phagocytes by surface modification with poly(L-lysine)-grafted poly(ethylene glycol) conjugate.
Article in Pharmaceutical research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Active fluctuations of cytoplasmic actomyosin networks facilitate dynein-driven transport.iScience · 2025Article
- Unraveling the in vivo fate of inhaled micro- and nanoplastics with PET imaging.The Science of the total environment · 2023Article
- TPGS Decorated Liposomes as Multifunctional Nano-Delivery Systems.Pharmaceutical research · 2023Review
- Engineering physical microenvironments to study innate immune cell biophysics.APL bioengineering · 2022Review
- Recent strategies towards the surface modification of liposomes: an innovative approach for different clinical applications.3 Biotech · 2020Review
- Macrophage-like THP-1 cells show effective uptake of silica nanoparticles carrying inactivated diphtheria toxoid for vaccination.Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology · 2020Article
- Interrogating Cellular Functions with Designer Janus Particles.Chemistry of materials : a publication of the American Chemical Society · 2017Article
- Effect of partial PEGylation on particle uptake by macrophages.Nanoscale · 2017Article
- Magnetic fingerprints of rolling cells for quantitative flow cytometry in whole blood.Scientific reports · 2016Article
- Molecular factors in dendritic cell responses to adsorbed glycoconjugates.Biomaterials · 2014Article
- Article
- Phagocytosis dynamics depends on target shape.Biophysical journal · 2013Article
- Facile Synthesis of PEGylated PLGA Nanoparticles Encapsulating Doxorubicin and its In Vitro Evaluation as Potent Drug Delivery Vehicle.Drug delivery and translational research · 2013Article
- Effect of the poly(ethylene glycol) (PEG) density on the access and uptake of particles by antigen-presenting cells (APCs) after subcutaneous administration.Molecular pharmaceutics · 2012Article
- Aptamer-conjugated polymeric nanoparticles for targeted cancer therapy.Drug delivery and translational research · 2012Article
- The Application of Microencapsulation Techniques in the Treatment of Endodontic and Periodontal Diseases.Pharmaceutics · 2011Article
- Multifunctional dendritic cell-targeting polymeric microparticles: engineering new vaccines for type 1 diabetes.Human vaccines · 2011Review
- Enhanced in vitro antiproliferative effects of EpCAM antibody-functionalized paclitaxel-loaded PLGA nanoparticles in retinoblastoma cells.Molecular vision · 2011Article
- PEGylated PLGA nanoparticles for the improved delivery of doxorubicin.Nanomedicine : nanotechnology, biology, and medicine · 2009Article
- Preparation of BMP-2 containing bovine serum albumin (BSA) nanoparticles stabilized by polymer coating.Pharmaceutical research · 2008Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe purpose of this study was to demonstrate specific receptor-mediated targeting of phagocytes by functional surface coatings of microparticles, shielding from nonspecific phagocytosis and allowing ligand-specific interactions via molecular recognition.
methodsCoatings of the comb polymer poly(L-lysine)-g-poly(ethylene glycol) (PLL-g-PEG) were investigated for potential to inhibit 1) nonspecific spreading of human blood-derived macrophages (MOs) and dendritic cells (DCs) on glass and 2) nonspecific phagocytosis of PLL-g-PEG-coated, carboxylated polystyrene (PS) or biodegradable poly(D,L-lactide-co-glycolide) (PLGA) microspheres. Coating was performed by adsorption of positively charged PLL-g-PEG on negatively charged microparticles or plasma-cleaned glass through electrostatic interaction. The feasibility of ligand-specific interactions was tested with a model ligand, RGD, conjugated to PEG chains of PLL-g-PEG to form PLL-g-PEG-RGD and compared with inactive ligand conjugate, PLL-g-PEG-RDG.
resultsCoatings with PLL-g-PEG largely impaired the adherence and spreading of MOs and DCs on glass. The repellent character of PLL-g-PEG coatings drastically reduced phagocytosis of coated PS and PLGA microparticles to 10% in presence of serum. With both MOs and DCs, we observed ligand-specific interactions with PLL-g-PEG-RGD coatings on glass and PS and PLGA microspheres. Ligand specificity was abolished when using inactive ligand conjugate PLL-g-PEG-RDG, whereas repellency of coating was maintained.
conclusionsCoatings of PLL-g-PEG-ligand conjugates provide a novel technology for ligand specific targeting of microspheres to MOs and DCs while reducing nonspecific phagocytosis.
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Registered trials
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