ArticleEMBO reports2003
Dynamics of the interaction between the insulin receptor and protein tyrosine-phosphatase 1B in living cells.
Article in EMBO reports, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 103 citations in OpenAlex.
- Increased O-GlcNAcylation promotes IGF-1 receptor/PhosphatidyI Inositol-3 kinase/Akt pathway in cervical cancer cells.Scientific reports · 2022Article
- Role of stilbenes against insulin resistance: A review.Food science & nutrition · 2021Review
- Junctional ER Organization Affects Mechanotransduction at Cadherin-Mediated Adhesions.Frontiers in cell and developmental biology · 2021Article
- Identification of insulin-sensitizing molecules acting by disrupting the interaction between the Insulin Receptor and Grb14.Scientific reports · 2017Article
- Phospho-Regulation of Soma-to-Axon Transcytosis of Neurotrophin Receptors.Developmental cell · 2017Article
- S-nitrosylation of endogenous protein tyrosine phosphatases in endothelial insulin signaling.Free radical biology & medicine · 2016Article
- UBC9-dependent association between calnexin and protein tyrosine phosphatase 1B (PTP1B) at the endoplasmic reticulum.The Journal of biological chemistry · 2015Article
- Protein-tyrosine phosphatase 1B substrates and metabolic regulation.Seminars in cell & developmental biology · 2015Review
- Bridging the gap: membrane contact sites in signaling, metabolism, and organelle dynamics.The Journal of cell biology · 2014Article
- Cellular biochemistry methods for investigating protein tyrosine phosphatases.Antioxidants & redox signaling · 2014Review
- Demonstration of a direct interaction between β2-adrenergic receptor and insulin receptor by BRET and bioinformatics.PloS one · 2014Article
- BRET Biosensor Analysis of Receptor Tyrosine Kinase Functionality.Frontiers in endocrinology · 2013Article
- Association of the protein-tyrosine phosphatase DEP-1 with its substrate FLT3 visualized by in situ proximity ligation assay.PloS one · 2013Article
- Setting Up a Bioluminescence Resonance Energy Transfer High throughput Screening Assay to Search for Protein/Protein Interaction Inhibitors in Mammalian Cells.Frontiers in endocrinology · 2012Article
- Regulation of signaling at regions of cell-cell contact by endoplasmic reticulum-bound protein-tyrosine phosphatase 1B.PloS one · 2012Article
- ER-bound protein tyrosine phosphatase PTP1B interacts with Src at the plasma membrane/substrate interface.PloS one · 2012Article
- A new highly efficient substrate-trapping mutant of protein tyrosine phosphatase 1B (PTP1B) reveals full autoactivation of the insulin receptor precursor.The Journal of biological chemistry · 2011Article
- Jak2 is a negative regulator of ubiquitin-dependent endocytosis of the growth hormone receptor.PloS one · 2011Article
- PTP1B regulates Eph receptor function and trafficking.The Journal of cell biology · 2010Article
- PTP1B: a double agent in metabolism and oncogenesis.Trends in biochemical sciences · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The dynamics of the interaction of the insulin receptor with a substrate-trapping mutant of protein-tyrosine phosphatase 1B (PTP1B) were monitored in living human embryonic kidney cells using bioluminescence resonance energy transfer (BRET). Insulin dose-dependently stimulates this interaction, which could be followed in real time for more than 30 minutes. The effect of insulin on the BRET signal could be detected at early time-points (30 seconds), suggesting that in intact cells the tyrosine-kinase activity of the insulin receptor is tightly controlled by PTP1B. Interestingly, the basal (insulin-independent) interaction of the insulin receptor with PTP1B was much weaker with a soluble form of the tyrosine-phosphatase than with the endoplasmic reticulum (ER)-targeted form. Inhibition of insulin-receptor processing using tunicamycin suggests that the basal interaction occurs during insulin-receptor biosynthesis in the ER. Therefore, localization of PTP1B in this compartment might be important for the regulation of insulin receptors during their biosynthesis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.