ArticleJournal of virology2003
Rapid localization of Gag/GagPol complexes to detergent-resistant membrane during the assembly of human immunodeficiency virus type 1.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 59 citations in OpenAlex.
- Neutral sphingomyelinase 2 is required for HIV-1 maturation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Conserved Interaction of Lentiviral Vif Molecules with HIV-1 Gag and Differential Effects of Species-Specific Vif on Virus Production.Journal of virology · 2017Article
- Effects of Membrane Charge and Order on Membrane Binding of the Retroviral Structural Protein Gag.Journal of virology · 2016Article
- Inhibition of HIV-1 assembly by coiled-coil domain containing protein 8 in human cells.Scientific reports · 2015Article
- Gag-Pol Transframe Domain p6* Is Essential for HIV-1 Protease-Mediated Virus Maturation.PloS one · 2015Article
- Basic residues in the matrix domain and multimerization target murine leukemia virus Gag to the virological synapse.Journal of virology · 2013Article
- Article
- Placement of leucine zipper motifs at the carboxyl terminus of HIV-1 protease significantly reduces virion production.PloS one · 2012Article
- Dynamic Association between HIV-1 Gag and Membrane Domains.Molecular biology international · 2012Article
- Gag induces the coalescence of clustered lipid rafts and tetraspanin-enriched microdomains at HIV-1 assembly sites on the plasma membrane.Journal of virology · 2011Article
- New insights into HIV assembly and trafficking.Physiology (Bethesda, Md.) · 2011Review
- HIV-1 envelope glycoprotein biosynthesis, trafficking, and incorporation.Journal of molecular biology · 2011Review
- Retroviral matrix and lipids, the intimate interaction.Retrovirology · 2011Review
- The cellular source for APOBEC3G's incorporation into HIV-1.Retrovirology · 2011Article
- The Role of Lipids in Retrovirus Replication.Viruses · 2010Article
- Relationships between plasma membrane microdomains and HIV-1 assembly.Biology of the cell · 2010Review
- Formation of the tRNALys packaging complex in HIV-1.FEBS letters · 2010Review
- A single amino acid substitution in HIV-1 reverse transcriptase significantly reduces virion release.Journal of virology · 2010Article
- Lipids and membrane microdomains in HIV-1 replication.Virus research · 2009Review
- The y271 and i274 amino acids in reverse transcriptase of human immunodeficiency virus-1 are critical to protein stability.PloS one · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During human immunodeficiency virus type 1 (HIV-1) assembly in HIV-1-transfected COS7 cells, almost all steady-state Gag/Gag and Gag/GagPol complexes are membrane bound. However, exposure to 1% Triton X-100 gives results indicating that while all Gag/GagPol complexes remain associated with the detergent-resistant membrane (DRM), only 30% of Gag/Gag complexes are associated with the DRM. Analysis of the localization of newly synthesized Gag/Gag and Gag/GagPol to the membrane indicates that after a 10-min pulse with radioactive [(35)S]Cys-[(35)S]Met, all newly synthesized Gag/GagPol is found at the DRM. Only 30% of newly synthesized Gag/Gag moves to the membrane, and at 0 min of chase, only 38% of this membrane-bound Gag/Gag is associated with the DRM. During the first 30 min of chase, most membrane-bound Gag/Gag moves to the DRM, while between 30 and 60 min of chase, there is a significant decrease in membrane-bound Gag/Gag and Gag/GagPol. Since the localization of newly synthesized Gag/Gag to the DRM and the interaction of GagPol with Gag both depend upon Gag multimerization, the rapid localization of GagPol to the DRM probably reflects the interaction of all newly synthesized GagPol with the first newly synthesized polymeric Gag to associate with the DRM.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.