ArticleEuropean biophysics journal : EBJ2003
Combined nanomanipulation by atomic force microscopy and UV-laser ablation for chromosomal dissection.
Article in European biophysics journal : EBJ, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 52 citations in OpenAlex.
- Multidisciplinary methodologies used in the study of cable bacteria.FEMS microbiology reviews · 2025Review
- On-site processing of single chromosomal DNA molecules using optically driven microtools on a microfluidic workbench.Scientific reports · 2021Article
- Microirradiation techniques in radiobiological research.Journal of biosciences · 2015Review
- Analysis of DNA double-strand break response and chromatin structure in mitosis using laser microirradiation.Nucleic acids research · 2010Article
- Application of dynamic impedance spectroscopy to atomic force microscopy.Science and technology of advanced materials · 2008Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nanomanipulation and nanoextraction on a scale close to and beyond the resolution limit of light microscopy is needed for many modern applications in biological research. For the manipulation of biological specimens a combined microscope allowing for ultraviolet (UV) microbeam laser manipulation together with manipulation by an atomic force microscope (AFM) was used. In a one-step procedure, human metaphase chromosomes were dissected optically by the UV-laser ablation and mechanically by AFM manipulation. With both methods, sub-400-nm cuts could be achieved routinely. Thus, the AFM is an indispensable tool for in situ quality control of nanomanipulation. However, already on this scale the dilation of the topographic AFM image due to the tip geometry can become significant. Therefore the AFM images were restored using a tip geometry obtained by a blind tip-reconstruction algorithm. Cross-sectional analysis of the restored image reveals a 380-nm-wide UV-laser cut and AFM cuts between 70 nm and 280 nm.
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Registered trials
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