ArticleMolecular biology of the cell2003
Tor kinases are in distinct membrane-associated protein complexes in Saccharomyces cerevisiae.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 127 papers.
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Who cites it
127 citing papers in PubMed, 234 citations in OpenAlex.
- Metabolic Adaptations Determine the Evolutionary Trajectory of TOR Signaling in Diverse Eukaryotes.Biomolecules · 2025Article
- Lipase activity of recombinant KmYJR107Wp and KmLIP3p enzymes expressed in Saccharomyces cerevisiae BY4742 from Kluyveromyces marxianus L2029.Journal, genetic engineering & biotechnology · 2024Article
- Sugar signals pedal the cell cycle!Frontiers in plant science · 2024Review
- Transcription by the Three RNA Polymerases under the Control of the TOR Signaling Pathway inBiomolecules · 2023Review
- Protocadherin gamma C3: a new player in regulating vascular barrier function.Neural regeneration research · 2023Review
- TOR complex 2 is a master regulator of plasma membrane homeostasis.The Biochemical journal · 2022Article
- Conserved Pib2 regions have distinct roles in TORC1 regulation at the vacuole.Journal of cell science · 2022Article
- Article
- Identification of defined structural elements within TOR2 kinase required for TOR complex 2 assembly and function inMolecular biology of the cell · 2022Article
- Article
- Heterozygous Mutations in Aromatic Amino Acid Synthesis Genes Trigger TOR Pathway Activation inmicroPublication biology · 2022Article
- Article
- Analysis of the TORC1 interactome reveals a spatially distinct function of TORC1 in mRNP complexes.The Journal of cell biology · 2021Article
- Review
- Ras, PI3K and mTORC2 - three's a crowd?Journal of cell science · 2020Review
- Regulation of mTORC2 Signaling.Genes · 2020Review
- Conserved Ark1-related kinases function in a TORC2 signaling network.Molecular biology of the cell · 2020Article
- Inhibition of TOR inCells · 2019Article
- The TOR Pathway Plays Pleiotropic Roles in Growth and Stress Responses of the Fungal PathogenGenetics · 2019Article
- Analysis of the roles of phosphatidylinositol-4,5-Molecular biology of the cell · 2019Article
67 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Tor1p and Tor2p kinases, targets of the immune-suppressive antibiotic rapamycin, are components of a highly conserved signaling network that couples nutrient availability and cell growth. To gain insight into the molecular basis underlying Tor-dependent signaling, we used cell fractionation and immunoaffinity chromatography to examine the physical environment of Tor2p. We found that the majority of Tor2p associates with a membrane-bound compartment along with at least four other proteins, Avo1p-Avo3p and Lst8p. Using immunogold electron microscopy, we observed that Tor2p, as well as Tor1p, localizes in punctate clusters to regions adjacent to the plasma membrane and within the cell interior, often in association with characteristic membranous tracks. Cell fractionation, coimmunoprecipitation, and immunogold electron microscopy experiments confirmed that Lst8 associates with both Tor2p as well as Tor1p at these membranous sites. In contrast, we find that Kog1, the yeast homologue of the mammalian Tor regulatory protein Raptor, interacts preferentially with Tor1p. These findings provide evidence for the existence of Tor signaling complexes that contain distinct as well as overlapping components. That these complexes colocalize to a membrane-bound compartment suggests an intimate relationship between membrane-mediated signaling and Tor activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.