ArticleMolecular biology of the cell2003
Pre-M phase-promoting factor associates with annulate lamellae in Xenopus oocytes and egg extracts.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 27 citations in OpenAlex.
- Cytoplasmic nucleoporin assemblage: the cellular artwork in physiology and disease.Nucleus (Austin, Tex.) · 2024Review
- Theileria parasites sequester host eIF5A to escape elimination by host-mediated autophagy.Nature communications · 2024Article
- Combined Exogenous Activation of Bovine Oocytes: Effects on Maturation-Promoting Factor, Mitogen-Activated Protein Kinases, and Embryonic Competence.International journal of molecular sciences · 2023Article
- Nucleoporin foci are stress-sensitive condensates dispensable for C. elegans nuclear pore assembly.The EMBO journal · 2023Article
- Emerin induces nuclear breakage inMolecular biology of the cell · 2018Article
- Hermes (Rbpms) is a Critical Component of RNP Complexes that Sequester Germline RNAs during Oogenesis.Journal of developmental biology · 2016Article
- CDC2/SPDY transiently associates with endoplasmic reticulum exit sites during oocyte maturation.BMC developmental biology · 2009Article
- Nuclear pore disassembly from endoplasmic reticulum membranes promotes Ca2+ signalling competency.The Journal of physiology · 2008Article
- Cdk1 and okadaic acid-sensitive phosphatases control assembly of nuclear pore complexes in Drosophila embryos.Molecular biology of the cell · 2005Article
- Two distinct Staufen isoforms in Xenopus are vegetally localized during oogenesis.RNA (New York, N.Y.) · 2004Article
- Localization and dynamics of Cdc2-cyclin B during meiotic reinitiation in starfish oocytes.Molecular biology of the cell · 2003Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have used complementary biochemical and in vivo approaches to study the compartmentalization of M phase-promoting factor (MPF) in prophase Xenopus eggs and oocytes. We first examined the distribution of MPF (Cdc2/CyclinB2) and membranous organelles in high-speed extracts of Xenopus eggs made during mitotic prophase. These extracts were found to lack mitochondria, Golgi membranes, and most endoplasmic reticulum (ER) but to contain the bulk of the pre-MPF pool. This pre-MPF could be pelleted by further centrifugation along with components necessary to activate it. On activation, Cdc2/CyclinB2 moved into the soluble fraction. Electron microscopy and Western blot analysis showed that the pre-MPF pellet contained a specific ER subdomain comprising "annulate lamellae" (AL): stacked ER membranes highly enriched in nuclear pores. Colocalization of pre-MPF with AL was demonstrated by anti-CyclinB2 immunofluorescence in prophase oocytes, in which AL are positioned close to the vegetal surface. Green fluorescent protein-CyclinB2 expressed in oocytes also localized at AL. These data suggest that inactive MPF associates with nuclear envelope components just before activation. This association may explain why nuclei and centrosomes stimulate MPF activation and provide a mechanism for targeting of MPF to some of its key substrates.
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Registered trials
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