ArticleMolecular biology of the cell2003
The fission yeast spo14+ gene encoding a functional homologue of budding yeast Sec12 is required for the development of forespore membranes.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 48 citations in OpenAlex.
- Sporulation: A response to starvation in the fission yeast Schizosaccharomyces pombe.MicrobiologyOpen · 2022Review
- Quick-Freeze, Deep-Etch Electron Microscopy Reveals the Characteristic Architecture of the Fission Yeast Spore.Journal of fungi (Basel, Switzerland) · 2020Article
- Kinesin-14 family proteins and microtubule dynamics defineJournal of cell science · 2020Article
- The fission yeast SPB component Dms1 is required to initiate forespore membrane formation and maintain meiotic SPB components.PloS one · 2018Article
- The exocytic Rabs Ypt3 and Ypt2 regulate the early step of biogenesis of the spore plasma membrane in fission yeast.Molecular biology of the cell · 2016Article
- The fission yeast spore is coated by a proteinaceous surface layer comprising mainly Isp3.Molecular biology of the cell · 2014Article
- A genome-wide screen for sporulation-defective mutants in Schizosaccharomyces pombe.G3 (Bethesda, Md.) · 2014Article
- Characterization of Schizosaccharomyces pombe copper transporter proteins in meiotic and sporulating cells.The Journal of biological chemistry · 2014Article
- The fission yeast synaptobrevin ortholog Syb1 plays an important role in forespore membrane formation and spore maturation.Eukaryotic cell · 2013Article
- Mfc1 is a novel copper transporter during meiosis.Communicative & integrative biology · 2012Article
- Endocytosis is essential for dynamic translocation of a syntaxin 1 orthologue during fission yeast meiosis.Molecular biology of the cell · 2011Article
- The fission yeast pleckstrin homology domain protein Spo7 is essential for initiation of forespore membrane assembly and spore morphogenesis.Molecular biology of the cell · 2011Article
- Roles of the DYRK kinase Pom2 in cytokinesis, mitochondrial morphology, and sporulation in fission yeast.PloS one · 2011Article
- Schizosaccharomyces pombe calmodulin, Cam1, plays a crucial role in sporulation by recruiting and stabilizing the spindle pole body components responsible for assembly of the forespore membrane.Eukaryotic cell · 2010Article
- A guaninine nucleotide exchange factor is a component of the meiotic spindle pole body in Schizosaccharomyces pombe.Molecular biology of the cell · 2010Article
- Role of septins in the orientation of forespore membrane extension during sporulation in fission yeast.Molecular and cellular biology · 2010Article
- Article
- Live observation of forespore membrane formation in fission yeast.Molecular biology of the cell · 2008Article
- Meiotic spindle pole bodies acquire the ability to assemble the spore plasma membrane by sequential recruitment of sporulation-specific components in fission yeast.Molecular biology of the cell · 2008Article
- Cation diffusion facilitator Cis4 is implicated in Golgi membrane trafficking via regulating zinc homeostasis in fission yeast.Molecular biology of the cell · 2008Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Schizosaccharomyces pombe spo14-B221 mutant was originally isolated as a sporulation-deficient mutant. However, the spo14(+) gene is essential for cell viability and growth. spo14(+) is identical to the previously characterized stl1(+) gene encoding a putative homologue of Saccharomyces cerevisiae Sec12, which is essential for protein transport from the endoplasmic reticulum (ER) to the Golgi apparatus. In the spo14 mutant cells, ER-like membranes were accumulated beneath the plasma membrane and the ER/Golgi shuttling protein Rer1 remained in the ER. Sec12 is a guanine nucleotide exchange factor for the Sar1 GTPase. Overproduction of psr1(+) coding for an S. pombe Sar1 homologue suppressed both the sporulation defect of spo14-B221 and cold-sensitive growth of newly isolated spo14-6 and spo14-7 mutants. These results indicate that Spo14 is involved in early steps of the protein secretory pathway. The spo14-B221 allele carries a single nucleotide change in the branch point consensus of the fifth intron, which reduces the abundance of the spo14 mRNA. During meiosis II, the forespore membrane was initiated near spindle pole bodies; however, subsequent extension of the membrane was arrested before its closure into a sac. We conclude that Spo14 is responsible for the assembly of the forespore membrane by supplying membrane vesicles.
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