ArticleProceedings of the National Academy of Sciences of the United States of America2003
The matrix attachment region in the Chinese hamster dihydrofolate reductase origin of replication may be required for local chromatid separation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 29 citations in OpenAlex.
- Advances in the Development and the Applications of Nonviral, Episomal Vectors for Gene Therapy.Human gene therapy · 2021Article
- Article
- In vivo mapping of arabidopsis scaffold/matrix attachment regions reveals link to nucleosome-disfavoring poly(dA:dT) tracts.The Plant cell · 2014Article
- Article
- Single-allele analysis of transcription kinetics in living mammalian cells.Nature methods · 2010Article
- Differential nuclear scaffold/matrix attachment marks expressed genes.Human molecular genetics · 2009Article
- A revisionist replicon model for higher eukaryotic genomes.Journal of cellular biochemistry · 2008Review
- Anchoring the genome.Genome biology · 2008Review
- Nuclear scaffold/matrix attached region modules linked to a transcription unit are sufficient for replication and maintenance of a mammalian episome.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- The human beta-globin replication initiation region consists of two modular independent replicators.Molecular and cellular biology · 2004Article
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3 authors at 1 institution in 1 country.
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Abstract
Centered in the Chinese hamster dihydrofolate reductase origin of replication is a prominent nuclear matrix attachment region (MAR). Indirect lines of evidence suggested that this MAR might be required for origin activation in early S phase. To test this possibility, we have deleted the MAR from a Chinese hamster ovary variant harboring a single copy of the dihydrofolate reductase locus. However, 2D gel replicon mapping shows that removal of the MAR has no significant effect either on the frequency or timing of initiation in this locus. Rather, fluorescence in situ hybridization studies on cells swollen under either neutral or alkaline conditions show that deletion of the MAR interferes with local separation of daughter chromatids. This surprising result provides direct genetic evidence that at least a subset of MARs performs an important biological function, possibly related to chromatid cohesion and separation.
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