ArticleProceedings of the National Academy of Sciences of the United States of America2003
Intein-mediated assembly of a functional beta-glucuronidase in transgenic plants.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 48 citations in OpenAlex.
- Split RNA switch orchestrates pre- and post-translational control to enable cell type-specific gene expression.Nature communications · 2025Article
- Real-Time, Non-Invasive Monitoring of Neuronal Differentiation Using Intein-Enabled Fluorescence Signal Translocation in Genetically Encoded Stem Cell-Based Biosensors.Advanced functional materials · 2024Article
- Inteins in Science: Evolution to Application.Microorganisms · 2020Review
- The application of gene splitting technique for controlling transgene flow in rice.Transgenic research · 2020Article
- Split intein-mediated selection of cells containing two plasmids using a single antibiotic.Nature communications · 2019Article
- EngineeredSaudi journal of biological sciences · 2019Article
- Biotechnological Applications of Protein Splicing.Current protein & peptide science · 2019Review
- Directed plant cell-wall accumulation of iron: embedding co-catalyst for efficient biomass conversion.Biotechnology for biofuels · 2016Article
- Seed-Specific Expression of Spider Silk Protein Multimers Causes Long-Term Stability.Frontiers in plant science · 2016Article
- Intein applications: from protein purification and labeling to metabolic control methods.The Journal of biological chemistry · 2014Review
- Article
- Gene-splitting technology: a novel approach for the containment of transgene flow in Nicotiana tabacum.PloS one · 2014Article
- The production of male-sterile wheat plants through split barnase expression is promoted by the insertion of introns and flexible peptide linkers.Transgenic research · 2013Article
- Native-sized spider silk proteins synthesized in planta via intein-based multimerization.Transgenic research · 2013Article
- Protein trans-splicing of multiple atypical split inteins engineered from natural inteins.PloS one · 2013Article
- Engineering a thermoregulated intein-modified xylanase into maize for consolidated lignocellulosic biomass processing.Nature biotechnology · 2012Article
- Reevaluation of the reliability and usefulness of the somatic homologous recombination reporter lines.The Plant cell · 2012Review
- Highly efficient and more general cis- and trans-splicing inteins through sequential directed evolution.The Journal of biological chemistry · 2011Article
- Reconstitution of glyphosate resistance from a split 5-enolpyruvyl shikimate-3-phosphate synthase gene in Escherichia coli and transgenic tobacco.Applied and environmental microbiology · 2007Article
- Functional analysis of the split Synechocystis DnaE intein in plant tissues by biolistic particle bombardment.Transgenic research · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The DnaE intein in Synechocystis sp. strain PCC6803 is the first and only naturally split intein that has been identified so far. It is capable of catalyzing a protein trans-splicing mechanism to assemble a mature protein from two separate precursors. Therefore, it is a powerful tool for protein modification and engineering. Inteins have not been identified, nor have intein-mediated protein splicing reactions been demonstrated, in plant cells. In this paper, we describe the use of the Ssp DnaE split intein in transgenic plants for reconstitution of a protein trans-splicing reaction. We have synthesized artificial genes that encode for N-terminal half (Int-n) and C-terminal half (Int-c) fragments of Ssp DnaE split intein and divided beta-glucuronidase (GUS) gene to encode GUS-n and GUS-c parts of the enzyme as reporter. The in-frame fusions of GUSn/Intn and Intc/GUSc were constructed and transfected into Arabidopsis. We have observed in vivo reassembly of functional beta-glucuronidase when both GUSn/Intn and Intc/GUSc constructs were introduced into the same Arabidopsis genome either by cotransformation or through genetic crossing, hereby signifying an intein-mediated protein trans-splicing mechanism reconstituted in plant cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.