ArticleThe EMBO journal2003
Higher plant myosin XI moves processively on actin with 35 nm steps at high velocity.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed, 184 citations in OpenAlex.
- Recessive antimorph alleles reveal novel functions of the Opaque1 myosin XI in maize.Plant physiology · 2026Article
- Unconventional vesicle transport driven by formin-actin-myosin working system during pollen germination.Science advances · 2026Article
- Dynamics of full-length Arabidopsis myosin XI and its involvement in actin remodeling.Plant & cell physiology · 2025Article
- Polarity sorting of actin filaments by motor-driven cargo transport.Biophysical journal · 2025Article
- Cytoskeleton as a generator of characteristic physical properties of plant cells: 'cell wall,' 'large vacuole,' and 'cytoplasmic streaming'.Biophysics and physicobiology · 2025Article
- Go with the flow - bulk transport by molecular motors.Journal of cell science · 2023Review
- A myosin XI adaptor, TAPE, is essential for pollen tube elongation in rice.Plant physiology · 2022Article
- Autoregulation and dual stepping mode of MYA2, an Arabidopsis myosin XI responsible for cytoplasmic streaming.Scientific reports · 2022Article
- Discovery of ultrafast myosin, its amino acid sequence, and structural features.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Myosin XI drives polarized growth by vesicle focusing and local enrichment of F-actin in Physcomitrium patens.Plant physiology · 2021Article
- Quantitative cell biology of tip growth in moss.Plant molecular biology · 2021Review
- Unravelling the molecular basis of the dominant negative effect of myosin XI tails on P-bodies.PloS one · 2021Article
- Characterization of ancestral myosin XI from Marchantia polymorpha by heterologous expression in Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2020Article
- Plant Lipid Bodies Traffic on Actin to Plasmodesmata Motorized by Myosin XIs.International journal of molecular sciences · 2020Article
- A cytorhabdovirus phosphoprotein forms mobile inclusions trafficked on the actin/ER network for viral RNA synthesis.Journal of experimental botany · 2019Article
- Actin Bundles in The Pollen Tube.International journal of molecular sciences · 2018Review
- Functional Diversity of Class XI Myosins in Arabidopsis thaliana.Plant & cell physiology · 2018Article
- Myosin XI-K is involved in root organogenesis, polar auxin transport, and cell division.Journal of experimental botany · 2018Article
- Kinesins and Myosins: Molecular Motors that Coordinate Cellular Functions in Plants.Annual review of plant biology · 2018Review
- Myosin-Driven Intracellular Transport.Cold Spring Harbor perspectives in biology · 2018Review
15 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High velocity cytoplasmic streaming is found in various plant cells from algae to angiosperms. We characterized mechanical and enzymatic properties of a higher plant myosin purified from tobacco bright yellow-2 cells, responsible for cytoplasmic streaming, having a 175 kDa heavy chain and calmodulin light chains. Sequence analysis shows it to be a class XI myosin and a dimer with six IQ motifs in the light chain-binding domains of each heavy chain. Electron microscopy confirmed these predictions. We measured its ATPase characteristics, in vitro motility and, using optical trap nanometry, forces and movement developed by individual myosin XI molecules. Single myosin XI molecules move processively along actin with 35 nm steps at 7 micro m/s, the fastest known processive motion. Processivity was confirmed by actin landing rate assays. Mean maximal force was approximately 0.5 pN, smaller than for myosin IIs. Dwell time analysis of beads carrying single myosin XI molecules fitted the ATPase kinetics, with ADP release being rate limiting. These results indicate that myosin XI is highly specialized for generation of fast processive movement with concomitantly low forces.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.