ArticleThe Biochemical journal2003
Characterization of the nutrient-sensing response unit in the human asparagine synthetase promoter.
Article in The Biochemical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 47 citations in OpenAlex.
- Article
- The Neuronal and Peripheral Expressed Membrane-Bound UNC93A Respond to Nutrient Availability in Mice.Frontiers in molecular neuroscience · 2017Article
- The C/ebp-Atf response element (CARE) location reveals two distinct Atf4-dependent, elongation-mediated mechanisms for transcriptional induction of aminoacyl-tRNA synthetase genes in response to amino acid limitation.Nucleic acids research · 2016Article
- CHOP induces activating transcription factor 5 (ATF5) to trigger apoptosis in response to perturbations in protein homeostasis.Molecular biology of the cell · 2013Article
- Asparagine synthetase: regulation by cell stress and involvement in tumor biology.American journal of physiology. Endocrinology and metabolism · 2013Review
- Heat shock factor 1 is inactivated by amino acid deprivation.Cell stress & chaperones · 2012Article
- ATF4-dependent transcription mediates signaling of amino acid limitation.Trends in endocrinology and metabolism: TEM · 2009Review
- Transcriptional induction of the human asparagine synthetase gene during the unfolded protein response does not require the ATF6 and IRE1/XBP1 arms of the pathway.The Biochemical journal · 2009Article
- Despite increased ATF4 binding at the C/EBP-ATF composite site following activation of the unfolded protein response, system A transporter 2 (SNAT2) transcription activity is repressed in HepG2 cells.The Journal of biological chemistry · 2008Article
- Deprivation of protein or amino acid induces C/EBPbeta synthesis and binding to amino acid response elements, but its action is not an absolute requirement for enhanced transcription.The Biochemical journal · 2008Article
- A feedback transcriptional mechanism controls the level of the arginine/lysine transporter cat-1 during amino acid starvation.The Biochemical journal · 2007Article
- Activation of the ATF3 gene through a co-ordinated amino acid-sensing response programme that controls transcriptional regulation of responsive genes following amino acid limitation.The Biochemical journal · 2007Article
- ATF2 is required for amino acid-regulated transcription by orchestrating specific histone acetylation.Nucleic acids research · 2007Article
- Alignment of the transcription start site coincides with increased transcriptional activity from the human asparagine synthetase gene following amino acid deprivation of HepG2 cells.The Journal of nutrition · 2006Article
- Characterization of the amino acid response element within the human sodium-coupled neutral amino acid transporter 2 (SNAT2) System A transporter gene.The Biochemical journal · 2006Article
- Asparagine synthetase chemotherapy.Annual review of biochemistry · 2006Review
- Amino-acid limitation induces transcription from the human C/EBPbeta gene via an enhancer activity located downstream of the protein coding sequence.The Biochemical journal · 2005Article
- Epigenetic changes in the repression and induction of asparagine synthetase in human leukemic cell lines.Leukemia · 2005Article
- Nutritional control of gene expression: how mammalian cells respond to amino acid limitation.Annual review of nutrition · 2005Review
- Methylation of the asparagine synthetase promoter in human leukemic cell lines is associated with a specific methyl binding protein.Oncogene · 2004Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Transcription from the human asparagine synthetase (A.S.) gene is increased in response to either amino acid (amino acid response) or glucose (endoplasmic reticulum stress response) deprivation. These two independent nutrient-sensing pathways converge on the same set of genomic cis -elements, referred to as nutrient sensing-response elements (NSREs) 1 and 2, within the A.S. promoter. The present report uses single-nucleotide mutagenesis to confirm that both NSRE-1 and NSRE-2 are absolutely required for gene activation and to identify the boundaries of each binding site. The core sequence of the NSRE-1 site is contained within nucleotides -68 to -60 and the NSRE-2 core sequence is within nucleotides -48 to -43. Through insertion or deletion of 5-10 nucleotides in the intervening sequence between NSRE-1 and NSRE-2, transient transfection studies with an A.S. promoter/reporter gene construct showed that the 11 bp distance between these two elements is critical. These results document that the optimal configuration is with both binding sites on the same side of the DNA helix, only one helical turn away from each other and the data provide support for the hypothesis that a larger multi-protein complex exists between the binding proteins for NSRE-1 and NSRE-2. The data also illustrate that the combination of NSRE-1 and NSRE-2, referred to as the nutrient-sensing response unit (NSRU), has enhancer activity in that it functions in an orientation- and position-independent manner, and conveys nutrient-dependent transcriptional control to a heterologous promoter.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.