ArticleProceedings of the National Academy of Sciences of the United States of America2003
Mutations that affect the ability of the vnd/NK-2 homeoprotein to regulate gene expression: transgenic alterations and tertiary structure.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 26 citations in OpenAlex.
- NK2.2/Vnd acts as a transcriptional activator of deadpan to maintain neuroblast identity in Drosophila embryos.Genes & genomics · 2026Article
- Major β cell-specific functions of NKX2.2 are mediated via the NK2-specific domain.Genes & development · 2023Article
- Cognate DNA Recognition by Engrailed Homeodomain Involves a Conformational Change Controlled via an Electrostatic-Spring-Loaded Latch.International journal of molecular sciences · 2022Article
- A Homeobox Transcription Factor Scarecrow (SCRO) Negatively Regulates Pdf Neuropeptide Expression through Binding an Identified cis-Acting Element in Drosophila melanogaster.Molecular neurobiology · 2020Article
- The alternative protein isoform NK2B, encoded by the vnd/NK-2 proneural gene, directly activates transcription and is expressed following the start of cells differentiation.Nucleic acids research · 2011Article
- Interactions of the acidic domain and SRF interacting motifs with the NKX3.1 homeodomain.Biochemistry · 2009Article
- How the Dorsal gradient works: insights from postgenome technologies.Proceedings of the National Academy of Sciences of the United States of America · 2008Review
- NOBOX homeobox mutation causes premature ovarian failure.American journal of human genetics · 2007Article
- Automatic discovery of cross-family sequence features associated with protein function.BMC bioinformatics · 2006Article
- An eh1-like motif in odd-skipped mediates recruitment of Groucho and repression in vivo.Molecular and cellular biology · 2005Article
- Hox genes and their candidate downstream targets in the developing central nervous system.Cellular and molecular neurobiology · 2005Review
- Identification and analysis of vnd/NK-2 homeodomain binding sites in genomic DNA.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Contextual interactions determine whether the Drosophila homeodomain protein, Vnd, acts as a repressor or activator.Nucleic acids research · 2005Article
- NMR structure of the noncytotoxic alpha-sarcin mutant Delta(7-22): the importance of the native conformation of peripheral loops for activity.Protein science : a publication of the Protein Society · 2004Article
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9 authors at 1 institution in 1 country.
Funding
Abstract
The importance in downstream target regulation of tertiary structure and DNA binding specificity of the protein encoded by the vndNK-2 homeobox gene is analyzed. The ectopic expression patterns of WT and four mutant vndNK-2 genes are analyzed together with expression of two downstream target genes, ind and msh, which are down-regulated by vndNK-2. Three mutants are deletions of conserved regions (i.e., tinman motif, acidic motif, and NK-2 box), and the fourth, Y54M vndNK-2, corresponds to a single amino acid residue replacement in the homeodomain. Of the four ectopically expressed mutant genes examined, only the Y54M mutation inactivates the ability of the vndNK-2 homeodomain protein to repress ind and msh. The acidic motif deletion mutant slightly reduced the ability of the protein to repress ind and msh. By contrast, both tinman and NK-2 box deletion mutants behaved as functional vndNK-2 genes in their ability to repress ind and msh. The NMR-determined tertiary structures of the Y54M vndNK-2 homeodomain, both free and bound to DNA, are compared with the WT analog. The only structural difference observed for the mutant homeodomain is in the complex with DNA and involved closer interaction of the methionine-54 with A2, rather than with C3 of the (-) strand of the DNA. This subtle change in the homeodomain-DNA complex resulted in modifications of binding affinities to DNA. These changes resulting from a single amino acid residue replacement constitute the molecular basis for the phenotypic alterations observed on ectopic expression of the Y54M vndNK-2 gene during embryogenesis.
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