ArticleNucleic acids research2003
RPA is an initiation factor for human chromosomal DNA replication.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 25 citations in OpenAlex.
- RPA1 protects DNA damage-induced PANoptosis in limb development.Science advances · 2025Article
- Genome-wide identification and characterisation of human DNA replication origins by initiation site sequencing (ini-seq).Nucleic acids research · 2016Article
- Structural and functional analysis of four non-coding Y RNAs from Chinese hamster cells: identification, molecular dynamics simulations and DNA replication initiation assays.BMC molecular biology · 2016Article
- The midblastula transition defines the onset of Y RNA-dependent DNA replication in Xenopus laevis.Molecular and cellular biology · 2011Article
- Dynamic interaction of Y RNAs with chromatin and initiation proteins during human DNA replication.Journal of cell science · 2011Article
- Article
- Clustering phenotype populations by genome-wide RNAi and multiparametric imaging.Molecular systems biology · 2010Article
- A conserved motif of vertebrate Y RNAs essential for chromosomal DNA replication.RNA (New York, N.Y.) · 2009Article
- BRCA1 and Tip60 determine the cellular response to ultraviolet irradiation through distinct pathways.The Journal of cell biology · 2008Article
- Functional requirement of noncoding Y RNAs for human chromosomal DNA replication.Molecular and cellular biology · 2006Article
- Visualization of bidirectional initiation of chromosomal DNA replication in a human cell free system.Nucleic acids research · 2005Article
- Silencing of chromatin assembly factor 1 in human cells leads to cell death and loss of chromatin assembly during DNA synthesis.Molecular and cellular biology · 2004Article
- XRCC3 deficiency results in a defect in recombination and increased endoreduplication in human cells.The EMBO journal · 2004Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The initiation of chromosomal DNA replication in human cell nuclei is not well understood because of its complexity. To allow investigation of this process on a molecular level, we have recently established a cell-free system that initiates chromosomal DNA replication in an origin-specific manner under cell cycle control in isolated human cell nuclei. We have now used fractionation and reconstitution experiments to functionally identify cellular factors present in a human cell extract that trigger initiation of chromosomal DNA replication in this system. Initial fractionation of a cytosolic extract indicates the presence of at least two independent and non-redundant initiation factors. We have purified one of these factors to homogeneity and identified it as the single-stranded DNA binding protein RPA. The prokaryotic single-stranded DNA binding protein SSB cannot substitute for RPA in the initiation of human chromosomal DNA replication. Antibodies specific for human RPA inhibit the initiation step of human chromosomal DNA replication in vitro. RPA is recruited to DNA replication foci and becomes phosphorylated concomitant with the initiation step in vitro. These data establish a direct functional role for RPA as an essential factor for the initiation of human chromosomal DNA replication.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.