ArticleClinical and diagnostic laboratory immunology2003
Inhibition of pneumococcal adherence to human nasopharyngeal epithelial cells by anti-PsaA antibodies.
Article in Clinical and diagnostic laboratory immunology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Article
- Molecular Characterization of Predominant Serotypes, Drug Resistance, and Virulence Genes ofFrontiers in microbiology · 2022Article
- Diverse Mechanisms of Protective Anti-Pneumococcal Antibodies.Frontiers in cellular and infection microbiology · 2022Review
- Pneumococcal surface adhesion A protein (PsaA) interacts with human Annexin A2 on airway epithelial cells.Virulence · 2021Article
- Self-assembled particulate PsaA as vaccine againstHeliyon · 2017Article
- Article
- Human antibodies to PhtD, PcpA, and Ply reduce adherence to human lung epithelial cells and murine nasopharyngeal colonization by Streptococcus pneumoniae.Infection and immunity · 2014Article
- Role of Pht proteins in attachment of Streptococcus pneumoniae to respiratory epithelial cells.Infection and immunity · 2014Article
- Absence of high molecular weight proteins 1 and/or 2 is associated with decreased adherence among non-typeable Haemophilus influenzae clinical isolates.Journal of medical microbiology · 2013Article
- Phenotypic, genomic, and transcriptional characterization of Streptococcus pneumoniae interacting with human pharyngeal cells.BMC genomics · 2013Article
- Transcriptional adaptation of pneumococci and human pharyngeal cells in the presence of a virus infection.BMC genomics · 2013Article
- Vaccine candidates PhtD and PhtE of Streptococcus pneumoniae are adhesins that elicit functional antibodies in humans.Vaccine · 2012Article
- Lipoproteins of bacterial pathogens.Infection and immunity · 2011Review
- Enhanced protection against nasopharyngeal carriage of Streptococcus pneumoniae elicited by oral multiantigen DNA vaccines delivered in attenuated Salmonella typhimurium.Molecular biology reports · 2011Article
- Immune responses to recombinant pneumococcal PsaA antigen delivered by a live attenuated Salmonella vaccine.Infection and immunity · 2010Article
- The laminin-binding protein Lbp from Streptococcus pyogenes is a zinc receptor.Journal of bacteriology · 2009Article
- Streptococcus adherence and colonization.Microbiology and molecular biology reviews : MMBR · 2009Review
- The metal homeostasis protein, Lsp, of Streptococcus pyogenes is necessary for acquisition of zinc and virulence.Infection and immunity · 2009Article
- Identification of Mannheimia haemolytica adhesins involved in binding to bovine bronchial epithelial cells.Infection and immunity · 2009Article
- Staphylococcus aureus deficient in lipidation of prelipoproteins is attenuated in growth and immune activation.Infection and immunity · 2005Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The role of pneumococcal (Pnc) surface adhesin A (PsaA) in the adherence of Streptococcus pneumoniae (pneumococcus) to host cells is not well defined. We examined the effect of anti-PsaA antibodies in an inhibition of adherence assay using Detroit 562 nasopharyngeal human epithelial cells. Rabbit polyclonal (Pab) anti-recombinant PsaA (rPsaA) sera, a purified mouse monoclonal antibody (MAb) (MAb 6F62G8E12), and 22 healthy adult sera with known anti-PsaA IgG levels (obtained by enzyme-linked immunosorbent assay) were evaluated for their abilities to inhibit Pnc adherence to confluent monolayers (measured as percent reduction in CFU counts compared to those of uninhibited controls). Pnc adherence was dependent on capsular phenotype (no or low adherence for opaque strains). With an inoculum of 10(4) to 10(5) bacteria/well, the mean +/- standard deviation count in controls was 163 +/- 32 CFU/well for transparent strains. Low adherence was observed for a PsaA-minus mutant even at higher inoculum doses. Mean percent inhibitions of adherence with Pab and MAb were 54 and 50%, respectively. Adult sera showed inhibition in a dose-response fashion with a range of 98 to 8%, depending on the serum anti-PsaA antibody concentration. Absorption of Pab with rPsaA restored Pnc adherence to control levels. Absorption of sera with a PsaA-minus mutant did not result in a significant decrease (P >0.05) of inhibition of adherence activity. Additionally, nearly 100% of Pnc adherence was inhibited by lipidated rPsaA at 2.5 micro g/ml. Our data support the argument that PsaA is an adhesin that mediates Pnc adherence to human nasopharyngeal cells. This functional assay may be useful in evaluating antibodies elicited in response to PsaA vaccination.
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