ArticleProceedings of the National Academy of Sciences of the United States of America2003
The spatial targeting and nuclear matrix binding domains of SRm160.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 63 citations in OpenAlex.
- A Prion-Like Domain in EBV EBNA1 Promotes Phase Separation and Enables SRRM1 Splicing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- TREX (transcription/export)-NP complex exerts a dual effect on regulating polymerase activity and replication of influenza A virus.PLoS pathogens · 2022Article
- An Arabidopsis Retention and Splicing complex regulates root and embryo development through pre-mRNA splicing.Plant physiology · 2022Article
- Novel γ-sarcoglycan interactors in murine muscle membranes.Skeletal muscle · 2022Article
- Cell-Extracellular Matrix Interactions Play Multiple Essential Roles in Aortic Arch Development.Circulation research · 2021Article
- HITS-CLIP reveals sex-differential RNA binding and alterative splicing regulation of SRm160 in Drosophila.Journal of molecular cell biology · 2019Article
- Generation and characterization of monoclonal antibodies that recognize human and murine supervillin protein isoforms.PloS one · 2018Article
- The Ultrastructural Signature of Human Embryonic Stem Cells.Journal of cellular biochemistry · 2017Article
- Next-generation profiling to identify the molecular etiology of Parkinson dementia.Neurology. Genetics · 2016Article
- Coordinated Dynamics of RNA Splicing Speckles in the Nucleus.Journal of cellular physiology · 2016Article
- Subnuclear domain proteins in cancer cells support the functions of RUNX2 in the DNA damage response.Journal of cell science · 2015Article
- Multifunctional RNA processing protein SRm160 induces apoptosis and regulates eye and genital development in Drosophila.Genetics · 2014Article
- Nuclear double-fluorescent reporter for in vivo and ex vivo analyses of biological transitions in mouse nuclei.Mammalian genome : official journal of the International Mammalian Genome Society · 2013Article
- Regulation of mRNA export by the PI3 kinase/AKT signal transduction pathway.Molecular biology of the cell · 2013Article
- Nuclear shape changes are induced by knockdown of the SWI/SNF ATPase BRG1 and are independent of cytoskeletal connections.PloS one · 2013Article
- The FF4 and FF5 domains of transcription elongation regulator 1 (TCERG1) target proteins to the periphery of speckles.The Journal of biological chemistry · 2012Article
- A U1-U2 snRNP interaction network during intron definition.Molecular and cellular biology · 2012Article
- Live cell imaging of the cancer-related transcription factor RUNX2 during mitotic progression.Journal of cellular physiology · 2011Article
- The human nucleolar protein FTSJ3 associates with NIP7 and functions in pre-rRNA processing.PloS one · 2011Article
- Mitotic bookmarking of genes: a novel dimension to epigenetic control.Nature reviews. Genetics · 2010Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Ser-Arg (SR)-related protein SRm160 is a coactivator of pre-mRNA splicing. It bridges splicing factors located at the 5' splice site, branch site, and 3' splice site. Recently, SRm160 has also been shown to be involved in mRNA export as part of an exon-junction complex. SRm160 is highly concentrated in splicing speckles but is also present in long branched intranuclear tracks connecting splicing speckles with sites at the nuclear lamina. In this study we identified domains of SRm160 important for spatial targeting within the nucleus and for binding to the nuclear matrix. Using a series of FLAG- and enhanced GFP-conjugated deletion mutants we found two contiguous sequences that independently target SRm160 to nuclear matrix sites at splicing speckled domains: amino acids 300-350 and 351-688. Constructs containing amino acids 300-350 were also targeted to sites peripheral to speckled domains where most mRNA originate subsequent to splicing. Sequences from the N-terminal domain localized proteins to the nuclear lamina near sites where mRNA leaves the nucleus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.