Evidence map›Paper›PMID 12624031›Full record

ArticleJournal of clinical microbiology2003

Detection and identification of Bartonella species pathogenic for humans by PCR amplification targeting the riboflavin synthase gene (ribC).

G Johnson, M Ayers, S C C McClure, S E Richardson, R Tellier

Open access · greenAbstract read
In one paragraph

Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.

0numbers the graph read from it
0cells of the map it votes in
49citing papers in PubMed
3.1field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

49 citing papers in PubMed, 120 citations in OpenAlex.

  1. Article
  2. Atypical Case of PresumedChildren (Basel, Switzerland) · 2026
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  5. Emerging microbes & infections · 2025
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  7. Cultivation ofJournal of veterinary research · 2025
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  8. Article
  9. Review
  10. Molecular Survey of Anaplasmataceae Agents,Pathogens (Basel, Switzerland) · 2025
    Article
  11. Molecular survey of Bartonella spp., Ehrlichia spp., Anaplasma spp. and hemoplasmas in small synanthropic mammals in urban areas of Brazil's northern Amazon region.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2025
    Article
  12. Bartonella spp. in bats from the Brazilian Amazon Forest.Veterinary research communications · 2024
    Article
  13. Article
  14. Article
  15. Article
  16. Molecular Survey ofPathogens (Basel, Switzerland) · 2022
    Article
  17. Article
  18. Article
  19. Laboratory Diagnosis of 37 Cases ofJournal of clinical microbiology · 2021
    Article
  20. Molecular Detection ofPathogens (Basel, Switzerland) · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

G JohnsonDivision of Microbiology, Hospital for Sick Children, University of Toronto. Cabbagetown Pet Clinic, Toronto, Ontario, Canada.
M Ayers
S C C McClure
S E Richardson
R Tellier
SickKids Foundation · CAHospital for Sick Children · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Several Bartonella species have now been implicated as human pathogens. The recovery of these fastidious organisms in the clinical microbiology laboratory remains difficult, and current methods are still relatively insensitive. Thus, the bartonellae are good candidates for detection by PCR. We have developed a PCR assay which uses a single primer pair targeting the riboflavin synthase gene (ribC) and detected six Bartonella species that have been implicated in human disease, B. henselae, B. quintana, B. bacilliformis, B. clarridgeiae, B. elizabethae, and B. vinsonii subsp. berkhoffii. Species identification is achieved simply by restriction enzyme digestion of the amplicon. This PCR assay appears to be specific for the Bartonella genus because it failed to amplify DNA from several other bacterial species.

Indexed as

Bacterial ProteinsBacterial Typing TechniquesBartonellaBartonella InfectionsHumansNucleotidyltransferasesPhosphotransferases (Alcohol Group Acceptor)Polymerase Chain ReactionSensitivity and SpecificityBacterial ProteinsNucleotidyltransferasesPhosphotransferases (Alcohol Group Acceptor)RibC protein, bacteria

Identifiers

PMID12624031
PMCPMC150319
OpenAlexW2118806681

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.