ArticleJournal of clinical microbiology2003
p24 Antigen detection assay modified with a booster step for diagnosis and monitoring of human immunodeficiency virus type 1 infection.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 94 citations in OpenAlex.
- Lack of longitudinal intrapatient correlation between p24 antigenemia and levels of human immunodeficiency virus (HIV) type 1 RNA in patients with chronic hiv infection during structured treatment interruptions.Journal of clinical microbiology · 2004Trial
- Translational opportunities in aptamer and nanobody lateral flow assays within the WHO REASSURED framework.Sensors & diagnostics · 2026Review
- Characterization of HIV humoral immunity during analytical treatment interruption.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2026Article
- Distinguishing active HIV-1 infection from vaccine-induced seropositivity in HIV vaccine trial participants.Science advances · 2025Article
- A click chemistry amplified nanopore assay for ultrasensitive quantification of HIV-1 p24 antigen in clinical samples.Nature communications · 2022Article
- Peptide-Based Vaccines for Hepatocellular Carcinoma: A Review of Recent Advances.Journal of hepatocellular carcinoma · 2021Review
- Ultra-low HIV-1 p24 detection limits with a bioelectronic sensor.Analytical and bioanalytical chemistry · 2020Article
- Femtogram Level Sensitivity achieved by Surface Engineered Silica Nanoparticles in the Early Detection of HIV Infection.Scientific reports · 2017Article
- Development of Monoclonal Antibodies against HIV-1 p24 Protein and Its Application in Colloidal Gold Immunochromatographic Assay for HIV-1 Detection.BioMed research international · 2016Article
- The Diagnosis of HIV Infection in Infants and Children.Iranian journal of pathology · 2016Review
- Increased Sensitivity of HIV-1 p24 ELISA Using a Photochemical Signal Amplification System.Journal of acquired immune deficiency syndromes (1999) · 2015Article
- Enhanced Sensitivity for Detection of HIV-1 p24 Antigen by a Novel Nuclease-Linked Fluorescence Oligonucleotide Assay.PloS one · 2015Article
- Dengue virus serotype 1 non-structural protein NS5 expression interferes with HIV replication in a CD4+ T-cell line.The American journal of tropical medicine and hygiene · 2014Article
- Two types of nanoparticle-based bio-barcode amplification assays to detect HIV-1 p24 antigen.Virology journal · 2012Article
- High level soluble expression, one-step purification and characterization of HIV-1 p24 protein.Virology journal · 2011Article
- Analysis of the optimal cut-point for HIV-p24 antigen testing to diagnose HIV infection in HIV-exposed children from resource-constrained settings.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2011Article
- An amperometric immunosensor based on a polyelectrolyte/gold magnetic nanoparticle supramolecular assembly--modified electrode for the determination of HIV p24 in serum.Molecules (Basel, Switzerland) · 2010Article
- Nanoparticle-based immunoassays for sensitive and early detection of HIV-1 capsid (p24) antigen.The Journal of infectious diseases · 2010Article
- Multisite comparison of anti-human immunodeficiency virus microbicide activity in explant assays using a novel endpoint analysis.Journal of clinical microbiology · 2009Article
- A highly sensitive and dynamic immunofluorescent cytometric bead assay for the detection of HIV-1 p24.Journal of virological methods · 2009Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We modified a p24 antigen enzyme-linked immunosorbent assay as a method for diagnosis and monitoring of human immunodeficiency virus type 1 (HIV-1) subtype E infection. This modified assay is based on the use of preheated immune complex dissociation combined with a booster step using a regular Vironostika HIV-1 p24 antigen assay (bioMerieux) to decrease the lower limit of p24 antigen detection from 10 pg/ml (lower limit achievable when using a regular p24 antigen assay) to 0.5 pg/ml (100 virions/ml) by the new method. The correlation between the values obtained by the HIV-1 RNA (Amplicor HIV-1 Monitor) assay and the p24 antigen assay modified with a booster step antigen assay in 160 frozen plasma samples with known viral load and 80 blind fresh plasma samples by Spearman rank were 0.671 (R(2) = 0.450; P < 0.01) and 0.782 (R(2) = 0.612; P < 0.01). During antiretroviral treatment, the change of p24 antigen level at >/=0.5 log correlated well with the level of HIV-1 in plasma. In order to improve the early diagnosis of HIV-1 infection in 121 infants born to HIV-1-infected mothers, a heat-denatured plasma p24 antigen assay modified with a booster step was compared with DNA-PCR and HIV RNA (nucleic acid sequence-based amplification) assays. The sensitivity of the antigen test modified with a booster step was similar to that of the HIV-1 RNA (NASBA QL) assay and better than that of the DNA-PCR assay (100 versus 61.90%) for subjects 1 to 2 months old. The overall results from this study might renew interest in p24 antigen detection as an easily affordable alternative method for diagnosis of HIV-1 infection and monitoring of disease progression in developing countries.
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