ArticleJournal of clinical microbiology2003
Diagnosis of Bartonella endocarditis by a real-time nested PCR assay using serum.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 104 citations in OpenAlex.
- Molecular Detection ofPathogens (Basel, Switzerland) · 2026Article
- Article
- Bartonella quintana Infection in Kidney Transplant Recipients from Donor Experiencing Homelessness, United States, 2022.Emerging infectious diseases · 2024Article
- Bartonella quintana Endocarditis in Persons Experiencing Homelessness, New York, New York, USA, 2020-2023.Emerging infectious diseases · 2024Article
- Molecular prevalence and genetic diversity of Bartonella spp. in stray cats of İzmir, Turkey.Parasites & vectors · 2022Article
- Pathogens That Cause Acute Febrile Illness Among Children and Adolescents in Burkina Faso, Madagascar, and Sudan.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2021Article
- Identification of an Emergent Pathogen, Bartonella vinsonii, Using Next-Generation Sequencing in a Patient With Culture-Negative Endocarditis.Journal of the Pediatric Infectious Diseases Society · 2021Article
- Article
- Bartonella Species, an Emerging Cause of Blood-Culture-Negative Endocarditis.Clinical microbiology reviews · 2017Review
- Intracranial Aneurysms From Presumed Infective Endocarditis: The Dilemma of Persistently Negative Cultures.The Neurohospitalist · 2016Article
- Isolation and characterization of Bartonella bacilliformis from an expatriate Ecuadorian.Journal of clinical microbiology · 2008Article
- Evaluation of an internally controlled real-time polymerase chain reaction assay targeting the groEL gene for the detection of Bartonella spp. DNA in patients with suspected cat-scratch disease.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2007Article
- From cat scratch disease to endocarditis, the possible natural history of Bartonella henselae infection.BMC infectious diseases · 2007Article
- Bartonella quintana endocarditis in dogs.Emerging infectious diseases · 2006Article
- Real-time PCR in clinical microbiology: applications for routine laboratory testing.Clinical microbiology reviews · 2006Review
- Diagnosis of cat scratch disease with detection of Bartonella henselae by PCR: a study of patients with lymph node enlargement.Journal of clinical microbiology · 2005Article
- Novel chemically modified liquid medium that will support the growth of seven bartonella species.Journal of clinical microbiology · 2005Article
- Potential limitations of the 16S-23S rRNA intergenic region for molecular detection of Bartonella species.Journal of clinical microbiology · 2005Article
- Bartonella vinsonii subsp. arupensis as an agent of blood culture-negative endocarditis in a human.Journal of clinical microbiology · 2005Article
- Bacterial zoonoses and infective endocarditis, Algeria.Emerging infectious diseases · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bartonella endocarditis is a severe disease for which blood cultures frequently remain negative. We tested three PCR assays by using specimens of serum sampled early during the disease from 43 patients diagnosed in our laboratory as having Bartonella endocarditis on the basis of serological, culture, and/or valvular molecular detection. We tested a two-step nested PCR (TSN-PCR), a one-step nested PCR (OSN-PCR) with a regular thermal cycler, and a one-step nested PCR with the LightCycler (LCN-PCR). These assays were performed with primers derived from the riboflavin synthase-encoding gene ribC, never before amplified in our laboratory. Due to contamination of negative controls, the results of the TSN-PCR were not interpretable, and this technique was no longer considered. The LCN-PCR had a specificity of 100% and a sensitivity of 58.1%, higher than those of the OSN-PCR (18.6%; P < 0.01) and prolonged blood culturing (7.1%; P < 0.01). The LCN-PCR results correlated strictly with those of other direct diagnostic tests, when available, and identified the causative species for six patients previously diagnosed on the basis of serological analysis only. The efficacy of the LCN-PCR was not influenced by antibiotics (P = 0.96) but was altered by prolonged storage of serum specimens at -20 degrees C (P = 0.04). Overall, the LCN-PCR is specific and more sensitive than traditional methods (i.e., culturing and/or PCR with EDTA-treated blood). It can easily be applied to the diagnosis of patients with suspected Bartonella endocarditis, especially when only serum is available.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.