ArticleApplied and environmental microbiology2003
PCR detection of virulence genes in Yersinia enterocolitica and Yersinia pseudotuberculosis and investigation of virulence gene distribution.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
61 citing papers in PubMed, 211 citations in OpenAlex.
- Epidemiology of pathogenic enterobacteria in humans, livestock, and peridomestic rodents in rural Madagascar.PloS one · 2014Trial
- Prevalence and Geographical Distribution of FoodborneAnimals : an open access journal from MDPI · 2026Review
- Development of a cost-effective LAMP method for screening and monitoring enteropathogenicScience in One Health · 2025Article
- Survey on the Occurrence of Zoonotic Bacterial Pathogens in the Feces of Wolves (Microorganisms · 2024Article
- Clinical Performance of the LiquidArrayDiagnostics (Basel, Switzerland) · 2024Article
- Laboratory investigation of causes of bovine abortion and stillbirth in the Republic of Korea, 2014-2020.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024Article
- Thyroid abscess associated with thyrotoxicosis caused by Yersinia enterocolitica subsp. palearctica in a patient with follicular adenoma of the thyroid gland.BMC infectious diseases · 2024Article
- EnteropathogenicPathogens (Basel, Switzerland) · 2024Article
- Article
- Controlled Experimental Infection in Pigs with a Strain of Yersinia enterocolitica Harboring Genetic Markers for Human Pathogenicity: Colonization and Stability.Infection and immunity · 2023Article
- One Health surveillance-A cross-sectoral detection, characterization, and notification of foodborne pathogens.Frontiers in public health · 2023Article
- Cold Enrichment Methods for the Detection of Foodborne Yersiniosis: Friend or Foe?Pathogens (Basel, Switzerland) · 2022Article
- Multiplex PCR method for differentiating highly pathogenic Yersinia enterocolitica and low pathogenic Yersinia enterocolitica, and Yersinia pseudotuberculosis.The Journal of veterinary medical science · 2021Article
- Bacterial Lighthouses-Real-Time Detection ofBiosensors · 2021Article
- Occurrence and Genetic Correlations ofPathogens (Basel, Switzerland) · 2021Article
- Development of IMBs-qPCR detection method for Yersinia enterocolitica based on the foxA gene.Archives of microbiology · 2021Article
- Presence and Characterization of Zoonotic Bacterial Pathogens in Wild Boar Hunting Dogs (Animals : an open access journal from MDPI · 2021Article
- Evidence of Antimicrobial Resistance and Presence of Pathogenicity Genes inPathogens (Basel, Switzerland) · 2021Article
- Poly(indole-5-carboxylic acid)/reduced graphene oxide/gold nanoparticles/phage-based electrochemical biosensor for highly specific detection of Yersinia pseudotuberculosis.Mikrochimica acta · 2021Article
- Prevalence, Virulence and Antimicrobial Susceptibility ofPathogens (Basel, Switzerland) · 2021Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PCR-based assays were developed for the detection of plasmid- and chromosome-borne virulence genes in Yersinia enterocolitica and Yersinia pseudotuberculosis, to investigate the distribution of these genes in isolates from various sources. The results of PCR genotyping, based on 5 virulence-associated genes of 140 strains of Y. enterocolitica, were compared to phenotypic tests, such as biotyping and serotyping, and to virulence plasmid-associated properties such as calcium-dependent growth at 37 degrees C and Congo red uptake. The specificity of the PCR results was validated by hybridization. Genotyping data correlated well with biotype data, and most biotypes resulted in (nearly) homogeneous genotypes for the chromosomal virulence genes (ystA, ystB, and ail); however, plasmid-borne genes (yadA and virF) were detected with variable efficiency, due to heterogeneity within the bacterial population for the presence of the virulence plasmid. Of the virulence genes, only ystB was present in biotype 1A; however, within this biotype, pathogenic and apathogenic isolates could not be distinguished based on the detection of virulence genes. Forty Y. pseudotuberculosis isolates were tested by PCR for the presence of inv, yadA, and lcrF. All isolates were inv positive, and 88% of the isolates contained the virulence plasmid genes yadA and lcrF. In conclusion, this study shows that genotyping of Yersinia spp., based on both chromosome- and plasmid-borne virulence genes, is feasible and informative and can provide a rapid and reliable genotypic characterization of field isolates.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.