ArticleApplied and environmental microbiology2003
A real-time PCR assay for the detection of Campylobacter jejuni in foods after enrichment culture.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 145 citations in OpenAlex.
- Culture-Based Standard Methods for the Isolation ofPolish journal of microbiology · 2024Review
- Campylobacteriosis: A rising threat in foodborne illnesses.Open veterinary journal · 2024Review
- Investigation of Trehalose Supplementation ImpactingVeterinary sciences · 2023Article
- Development of a Lateral Flow Strip-Based Recombinase-Aided Amplification for ActiveFrontiers in microbiology · 2022Article
- The Sensitivity and Specificity of Loop-Mediated Isothermal Amplification and PCR Methods in Detection of Foodborne Microorganisms: A Systematic Review and Meta-Analysis.Iranian journal of public health · 2021Review
- Real-time quantitative PCR assay development and application for assessment of agricultural surface water and various fecal matter for prevalence of Aliarcobacter faecis and Aliarcobacter lanthieri.BMC microbiology · 2020Article
- A sensitive gold-nanorods-based nanobiosensor for specific detection of Campylobacter jejuni and Campylobacter coli.Journal of nanobiotechnology · 2019Article
- Generation and characterization of a novel recombinant scFv antibody specific for Campylobacter jejuni.Applied microbiology and biotechnology · 2018Article
- Aetiology of diarrhoeal disease and evaluation of viral-bacterial coinfection in children under 5 years old in China: a matched case-control study.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2016Article
- Detection ofFood science and biotechnology · 2016Article
- Environmental monitoring of waterborne Campylobacter: evaluation of the Australian standard and a hybrid extraction-free MPN-PCR method.Frontiers in microbiology · 2015Article
- Detection, identification and quantification of Campylobacter jejuni, coli and lari in food matrices all at once using multiplex qPCR.Gut pathogens · 2014Article
- Development of defined microbial population standards using fluorescence activated cell sorting for the absolute quantification of S. aureus using real-time PCR.Molecular biotechnology · 2012Article
- Rapid identification and quantification of Campylobacter coli and Campylobacter jejuni by real-time PCR in pure cultures and in complex samples.BMC microbiology · 2011Article
- Comparative analysis of cultural isolation and PCR based assay for detection of campylobacter jejuni in food and faecal samples.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2011Article
- A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats.Journal of veterinary science · 2009Article
- Comparison of loop-mediated isothermal amplification assay and conventional culture methods for detection of Campylobacter jejuni and Campylobacter coli in naturally contaminated chicken meat samples.Applied and environmental microbiology · 2009Article
- Real-time PCR method for detection of pathogenic Yersinia enterocolitica in food.Applied and environmental microbiology · 2008Article
- Real-time PCR approach for detection of environmental sources of Campylobacter strains colonizing broiler flocks.Applied and environmental microbiology · 2008Article
- Comparative, collaborative, and on-site validation of a TaqMan PCR method as a tool for certified production of fresh, campylobacter-free chickens.Applied and environmental microbiology · 2006Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A real-time PCR assay was developed for the quantitative detection of Campylobacter jejuni in foods after enrichment culture. The specificity of the assay for C. jejuni was demonstrated with a diverse range of Campylobacter species, related organisms, and unrelated genera. The assay had a linear range of quantification over six orders of magnitude, and the limit of detection was approximately 12 genome equivalents. The assay was used to detect C. jejuni in both naturally and artificially contaminated food samples. Ninety-seven foods, including raw poultry meat, offal, raw shellfish, and milk samples, were enriched in blood-free Campylobacter enrichment broth at 37 degrees C for 24 h, followed by 42 degrees C for 24 h. Enrichment cultures were subcultured to Campylobacter charcoal-cefoperazone-deoxycholate blood-free selective agar, and presumptive Campylobacter isolates were identified with phenotypic methods. DNA was extracted from enrichment cultures with a rapid lysis method and used as the template in the real-time PCR assay. A total of 66 samples were positive for C. jejuni by either method, with 57 samples positive for C. jejuni by subculture to selective agar medium and 63 samples positive in the real-time PCR assay. The results of both methods were concordant for 84 of the samples. The total time taken for detection from enrichment broth samples was approximately 3 h for the real-time PCR assay, with the results being available immediately at the end of PCR cycling, compared to 48 h for subculture to selective agar. This assay significantly reduces the total time taken for the detection of C. jejuni in foods and is an important model for other food-borne pathogens.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.