ArticleGenetics2003
piggyBac-based insertional mutagenesis and enhancer detection as a tool for functional insect genomics.
Article in Genetics, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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76 citing papers in PubMed.
- Random Insertion Reporter Gimmicks Powered by Cut-and-Paste DNA Transposons.Biomedicines · 2025Review
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- Changes in the cellular makeup of motor patterning circuits drive courtship song evolution in Drosophila.Current biology : CB · 2024Article
- CabioRxiv : the preprint server for biology · 2024Article
- Changes in the cellular makeup of motor patterning circuits drive courtship song evolution inbioRxiv : the preprint server for biology · 2024Article
- An Optimized Small-Scale Rearing System to Support Embryonic Microinjection Protocols for Western Corn Rootworm,Insects · 2023Article
- A conditional strategy for cell-type-specific labeling of endogenous excitatory synapses inCell reports methods · 2023Article
- Evolution ofInsects · 2023Article
- SericinInsects · 2023Article
- CRISPR-/Cas9-Mediated Precise and Efficient Genome Editing in Drosophila.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Genetic and viral approaches to record or manipulate neurons in insects.Current opinion in insect science · 2021Review
- A piggyBac-mediated transgenesis system for the temporary expression of CRISPR/Cas9 in rice.Plant biotechnology journal · 2021Article
- Phosphatase of Regenerating Liver-1 Selectively Times Circadian Behavior in Darkness via Function in PDF Neurons and Dephosphorylation of TIMELESS.Current biology : CB · 2021Article
- Characterizing Repeats in Two Whole-Genome Amplification Methods in the Reniform Nematode Genome.International journal of genomics · 2021Article
- The hAT-family transposable element, hopper, from Bactrocera dorsalis is a functional vector for insect germline transformation.BMC genetics · 2020Article
- Daughterless, theDisease models & mechanisms · 2020Article
- Endogenous Tagging Reveals Differential Regulation of CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Latrophilins: A Neuro-Centric View of an Evolutionary Conserved Adhesion G Protein-Coupled Receptor Subfamily.Frontiers in neuroscience · 2019Article
- Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing.Journal of visualized experiments : JoVE · 2018Article
- The neurobiological basis of sleep: Insights from Drosophila.Neuroscience and biobehavioral reviews · 2018Review
16 more citing papers are in PubMed but not listed here.
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5 authors.
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Abstract
Transposon mutagenesis provides a fundamental tool for functional genomics. Here we present a non-species-specific, combined enhancer detection and binary expression system based on the transposable element piggyBac: For the different components of this insertional mutagenesis system, we used widely applicable transposons and distinguishable broad-range transformation markers, which should enable this system to be operational in nonmodel arthropods. In a pilot screen in Drosophila melanogaster, piggyBac mutator elements on the X chromosome were mobilized in males by a Hermes-based jumpstarter element providing piggyBac transposase activity under control of the alpha1-tubulin promoter. As primary reporters in the piggyBac mutator elements, we employed the heterologous transactivators GAL4delta or tTA. To identify larval and adult enhancer detectors, strains carrying UASp-EYFP or TRE-EYFP as secondary reporter elements were used. Tissue-specific enhancer activities were readily observed in the GAL4delta/UASp-based systems, but only rarely in the tTA/TRE system. Novel autosomal insertions were recovered with an average jumping rate of 80%. Of these novel insertions, 3.8% showed homozygous lethality, which was reversible by piggyBac excision. Insertions were found in both coding and noncoding regions of characterized genes and also in noncharacterized and non-P-targeted CG-number genes. This indicates that piggyBac will greatly facilitate the intended saturation mutagenesis in Drosophila.
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